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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
20
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pubmed:dateCreated |
1992-8-14
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pubmed:databankReference |
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pubmed:abstractText |
Guanine nucleotide-binding proteins (G proteins) mediate signals between serotonin receptors and adenylate cyclase in Schistosoma mansoni. A bovine Gs alpha cDNA probe was used to isolate a cDNA clone, SG12, encoding the entire alpha-subunit of a G protein of S. mansoni. The cDNA is 1897 base pairs long, contains an open reading frame of 1137 base pairs, and codes for a deduced protein of 379 amino acids. The putative protein encoded by the clone has an exact amino acid match with bovine Gs alpha of 65% and a 78% match when conserved amino acid substitutions are considered. In contrast, the exact and conserved matches of the schistosome alpha-subunit with bovine Gi are 41 and 61%, respectively. A comparison of the deduced amino acid sequence of SG12 with a variety of different G alpha proteins indicates that all the major structural features characteristic of a Gs alpha protein are present in the S. mansoni gene. The schistosome clone contains the putative site for ADP-ribosylation by cholera toxin found in Gs alpha but does not contain the ADP-ribosylation site for pertussis toxin present in Gi alpha. The amino acids are completely conserved at the GTP-binding sites. On a Northern blot, the cDNA hybridizes to a major band of 3.1 kilobases in RNA from adult schistosomes. The message appears to be absent in miracidia and cercariae, but a faint 3.1-kilobase band is visible in the early schistosomule stage preceding adulthood. This evidence, when added to previous biochemical data, indicates that the expression of this gene is developmentally controlled.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
267
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
14504-8
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:1378444-Amino Acid Sequence,
pubmed-meshheading:1378444-Animals,
pubmed-meshheading:1378444-Antisense Elements (Genetics),
pubmed-meshheading:1378444-Base Sequence,
pubmed-meshheading:1378444-Cattle,
pubmed-meshheading:1378444-Cloning, Molecular,
pubmed-meshheading:1378444-DNA,
pubmed-meshheading:1378444-DNA Probes,
pubmed-meshheading:1378444-Drosophila,
pubmed-meshheading:1378444-GTP-Binding Proteins,
pubmed-meshheading:1378444-Gene Library,
pubmed-meshheading:1378444-Macromolecular Substances,
pubmed-meshheading:1378444-Male,
pubmed-meshheading:1378444-Molecular Sequence Data,
pubmed-meshheading:1378444-Molecular Weight,
pubmed-meshheading:1378444-Oligodeoxyribonucleotides,
pubmed-meshheading:1378444-RNA,
pubmed-meshheading:1378444-RNA Probes,
pubmed-meshheading:1378444-Restriction Mapping,
pubmed-meshheading:1378444-Schistosoma mansoni,
pubmed-meshheading:1378444-Sequence Homology, Nucleic Acid
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pubmed:year |
1992
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pubmed:articleTitle |
Cloning and characterization of a cDNA coding for the alpha-subunit of a stimulatory G protein from Schistosoma mansoni.
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pubmed:affiliation |
Department of Pharmacology, Stanford University School of Medicine, California 94305.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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