Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1992-8-20
pubmed:abstractText
P-selectin (CD62), formerly called GMP-140 or PADGEM, is a membrane protein located in secretory storage granules of platelets and endothelial cells. To study the mechanisms responsible for the targeting of P-selectin to storage granules, we transfected its cDNA into COS-7 and CHO-K1 cells, which lack a regulated exocytic pathway, or into AtT20 cells, which are capable of regulated secretion. P-selectin was expressed on the plasma membrane of COS-7 and CHO-K1 cells but was concentrated in storage granules of AtT20 cells. Immunogold electron microscopy indicated that the electron-dense granules containing P-selectin in AtT20 cells also stored the endogenous soluble hormone ACTH. Activation of AtT20 cells with 8-Br-cAMP increased the surface expression of P-selectin, consistent with agonist-induced fusion of granule membranes with the plasma membrane. Deletion of the last 23 amino acids of the 35-residue cytoplasmic domain resulted in delivery of P-selectin to the plasma membrane of AtT20 cells. Replacement of the cytoplasmic tail of tissue factor, a plasma membrane protein, with the cytoplasmic domain of P-selectin redirected the chimeric molecule to granules. We conclude that the cytoplasmic domain of P-selectin is both necessary and sufficient for sorting of membrane proteins into the regulated pathway of secretion.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1378326-1663373, http://linkedlifedata.com/resource/pubmed/commentcorrection/1378326-1689464, http://linkedlifedata.com/resource/pubmed/commentcorrection/1378326-1701178, http://linkedlifedata.com/resource/pubmed/commentcorrection/1378326-1718051, http://linkedlifedata.com/resource/pubmed/commentcorrection/1378326-1848556, http://linkedlifedata.com/resource/pubmed/commentcorrection/1378326-1906810, http://linkedlifedata.com/resource/pubmed/commentcorrection/1378326-1968060, http://linkedlifedata.com/resource/pubmed/commentcorrection/1378326-1985919, http://linkedlifedata.com/resource/pubmed/commentcorrection/1378326-1988154, http://linkedlifedata.com/resource/pubmed/commentcorrection/1378326-2120038, http://linkedlifedata.com/resource/pubmed/commentcorrection/1378326-2175900, http://linkedlifedata.com/resource/pubmed/commentcorrection/1378326-2209556, http://linkedlifedata.com/resource/pubmed/commentcorrection/1378326-2257624, http://linkedlifedata.com/resource/pubmed/commentcorrection/1378326-2391371, http://linkedlifedata.com/resource/pubmed/commentcorrection/1378326-2411738, http://linkedlifedata.com/resource/pubmed/commentcorrection/1378326-2460342, http://linkedlifedata.com/resource/pubmed/commentcorrection/1378326-2463989, http://linkedlifedata.com/resource/pubmed/commentcorrection/1378326-2466574, http://linkedlifedata.com/resource/pubmed/commentcorrection/1378326-2467701, http://linkedlifedata.com/resource/pubmed/commentcorrection/1378326-2470733, http://linkedlifedata.com/resource/pubmed/commentcorrection/1378326-2472431, http://linkedlifedata.com/resource/pubmed/commentcorrection/1378326-2473156, http://linkedlifedata.com/resource/pubmed/commentcorrection/1378326-2541923, http://linkedlifedata.com/resource/pubmed/commentcorrection/1378326-2557062, http://linkedlifedata.com/resource/pubmed/commentcorrection/1378326-2583113, http://linkedlifedata.com/resource/pubmed/commentcorrection/1378326-2663880, http://linkedlifedata.com/resource/pubmed/commentcorrection/1378326-2719931, http://linkedlifedata.com/resource/pubmed/commentcorrection/1378326-2808334, http://linkedlifedata.com/resource/pubmed/commentcorrection/1378326-2821010, http://linkedlifedata.com/resource/pubmed/commentcorrection/1378326-2836400, http://linkedlifedata.com/resource/pubmed/commentcorrection/1378326-2941452, http://linkedlifedata.com/resource/pubmed/commentcorrection/1378326-2945253, http://linkedlifedata.com/resource/pubmed/commentcorrection/1378326-3017997, http://linkedlifedata.com/resource/pubmed/commentcorrection/1378326-3031082, http://linkedlifedata.com/resource/pubmed/commentcorrection/1378326-3087627, http://linkedlifedata.com/resource/pubmed/commentcorrection/1378326-3194899, http://linkedlifedata.com/resource/pubmed/commentcorrection/1378326-3297348, http://linkedlifedata.com/resource/pubmed/commentcorrection/1378326-3318877, http://linkedlifedata.com/resource/pubmed/commentcorrection/1378326-3534077, http://linkedlifedata.com/resource/pubmed/commentcorrection/1378326-3545499, http://linkedlifedata.com/resource/pubmed/commentcorrection/1378326-3670292, http://linkedlifedata.com/resource/pubmed/commentcorrection/1378326-6279313, http://linkedlifedata.com/resource/pubmed/commentcorrection/1378326-6381508, http://linkedlifedata.com/resource/pubmed/commentcorrection/1378326-6935293
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1059-1524
pubmed:author
pubmed:issnType
Print
pubmed:volume
3
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
309-21
pubmed:dateRevised
2010-9-7
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Cytoplasmic domain of P-selectin (CD62) contains the signal for sorting into the regulated secretory pathway.
pubmed:affiliation
Department of Medicine, St. Francis Medical Research Institute, University of Oklahoma Health Sciences Center, Oklahoma City.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't