Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
|
pubmed:dateCreated |
1992-7-31
|
pubmed:abstractText |
Human placental trophoblasts, fibroblasts and the trophoblast-derived malignant cell JAR are potent producers of interferons (IFNs) when stimulated with Sendai virus. The three cell lines produced different levels and compositions of IFN-alpha subtypes and IFN-beta. Anti-IFN globulins, Cibacron Blue F3GA and Concanavalin A were covalently immobilized on pressure-stable, macroporous polymeric matrices derivatized with vinyl sulphone (HEMA-BIO 1000 VS and HEMA 1000 VS). These supports were packed in biocompatible PEEK columns and were coupled with switching valves, to develop a tandem high-performance affinity chromatographic (HPAC) method for the isolation, purification and biochemical characterization of the IFNs produced in Sendai virus-stimulated human placental trophoblasts, fibroblasts and trophoblast-derived malignant cell, JAR, cultures. Silver-stained SDS-PAGE and gel densitometric analysis revealed the purity of the purified proteins to be between 94 and 98%. Specific activities of the purified IFNs ranged between 0.37-2.76 x 10(8) IU/mg of protein with cumulative recoveries between 90 and 92.2%. The purified IFN components exhibited quantitatively different antiviral activities in human and bovine cell lines. The utility of the tandem method for the purification and characterization of human type 1 IFNs produced from other cell lines are also discussed.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Jun
|
pubmed:issn |
0032-7484
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
22
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
105-21
|
pubmed:dateRevised |
2004-11-17
|
pubmed:meshHeading |
pubmed-meshheading:1377824-Chromatography, Affinity,
pubmed-meshheading:1377824-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:1377824-Female,
pubmed-meshheading:1377824-Fibroblasts,
pubmed-meshheading:1377824-Humans,
pubmed-meshheading:1377824-Interferons,
pubmed-meshheading:1377824-Placenta,
pubmed-meshheading:1377824-Pregnancy,
pubmed-meshheading:1377824-Trophoblasts
|
pubmed:year |
1992
|
pubmed:articleTitle |
Isolation, purification and biochemical characterization of human placental interferons by tandem high-performance affinity chromatography.
|
pubmed:affiliation |
Department of Virus and Cancer, Danish Cancer Society, Aarhus.
|
pubmed:publicationType |
Journal Article
|