Linked Life Data

13775643

Source:http://linkedlifedata.com/resource/pubmed/id/13775643

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1961-12-1
pubmed:abstractText
Localized membrane current and potential measurements were made on the squid giant axon in voltage clamp experiments. Spatial control of potential was impaired by the use of axial current supplying electrodes with surface resistance greater than 20 ohms for a centimeter length of axon. No region of membrane which was indeed subjected to a potential step showed more than one inward current peak. Other patterns were results of space clamp failure. Membrane current and potential patterns during space clamp failure were approximately reproduced in computations on a model containing two membrane patches obeying the equations of Hodgkin and Huxley. Non-uniformities in the axon or electrodes are not necessary for non-uniform electrical behavior. An extension of the core conductor model which includes the axial wire and external solution has been analyzed. The space constant of electrotonic spread is less than 0.5 mm with a usable electrode. Errors of about 5 per cent are introduced by ignoring the external solution. Resistance between the membrane and the control electrodes reduces the control and a few ohm cm(2) could lead to serious errors in interpretation.
pubmed:commentsCorrections
pubmed:keyword
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
OM
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0006-3495
pubmed:author
pubmed:issnType
Print
pubmed:volume
1
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
161-202
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1960
pubmed:articleTitle
Analysis of certain errors in squid axon voltage clamp measurements.
pubmed:publicationType
Journal Article