Linked Life Data

13766855

Source:http://linkedlifedata.com/resource/pubmed/id/13766855

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1961-12-1
pubmed:abstractText
A new procedure is described for silver impregnation of thin sections for electron microscopy. Sections of various tissues, fixed in OsO(4) and embedded in methacrylate, were treated with an ammoniacal silver solution, directly or after oxidation with periodic acid or hydrogen peroxide. After OsO(4) fixation all cellular membranous systems exhibit a primary argentaffinity probably due to the reduction of ammoniacal silver solution by the reduced osmium bound to unsaturated lipids. Bleaching the sections with hydrogen peroxide removes the argentaffinity of protoplasmic structures. Treatment of the sections with periodic acid results in decreased argentaffinity of protoplasmic components while the argentaffinity of metaplasmic structures is greatly enhanced. The latter procedure appears particularly useful for enhancing the contrast of basement membranes.
pubmed:commentsCorrections
pubmed:keyword
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
OM
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0095-9901
pubmed:author
pubmed:issnType
Print
pubmed:volume
9
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
121-33
pubmed:dateRevised
2010-6-22
pubmed:meshHeading
pubmed:year
1961
pubmed:articleTitle
Silver impregnation of ultrathin sections for electron microscopy.
pubmed:publicationType
Journal Article