|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
6
|
|
pubmed:dateCreated |
1992-7-21
|
|
pubmed:databankReference |
|
|
pubmed:abstractText |
The adhesive interaction between circulating lymphocytes and the high endothelial venules (HEV) of lymph nodes (LN) is mediated by lymphocyte L-selectin, a member of the selectin family of cell adhesion proteins. Previous work has identified a sulfated 50 kd glycoprotein (Sgp50) as an HEV ligand for L-selectin. We now report the purification of this glycoprotein and the utilization of the derived N-terminal amino acid sequence to clone a cDNA. The predicted sequence reveals a novel, mucin-like molecule containing two serine/threonine-rich domains. The mRNA encoding this glycoprotein is preferentially expressed in LN. Antibodies against predicted peptides immunoprecipitate Sgp50 and stain the apical surface of LN HEV. These results thus define a tissue-specific mucin-like endothelial glycoprotein that appears to function as a scaffold that presents carbohydrates to the L-selectin lectin domain.
|
|
pubmed:grant |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jun
|
|
pubmed:issn |
0092-8674
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
12
|
|
pubmed:volume |
69
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
927-38
|
|
pubmed:dateRevised |
2007-11-14
|
|
pubmed:meshHeading |
pubmed-meshheading:1376638-Amino Acid Sequence,
pubmed-meshheading:1376638-Animals,
pubmed-meshheading:1376638-Base Sequence,
pubmed-meshheading:1376638-Blotting, Northern,
pubmed-meshheading:1376638-Cell Adhesion,
pubmed-meshheading:1376638-Cell Adhesion Molecules,
pubmed-meshheading:1376638-Cloning, Molecular,
pubmed-meshheading:1376638-DNA,
pubmed-meshheading:1376638-Genes,
pubmed-meshheading:1376638-L-Selectin,
pubmed-meshheading:1376638-Ligands,
pubmed-meshheading:1376638-Mice,
pubmed-meshheading:1376638-Molecular Sequence Data,
pubmed-meshheading:1376638-Mucins,
pubmed-meshheading:1376638-Oligodeoxyribonucleotides,
pubmed-meshheading:1376638-Protein Conformation
|
|
pubmed:year |
1992
|
|
pubmed:articleTitle |
An endothelial ligand for L-selectin is a novel mucin-like molecule.
|
|
pubmed:affiliation |
Department of Immunobiology, Genentech, Inc,, South San Francisco, California 94080.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|
