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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1-2
|
| pubmed:dateCreated |
1992-7-14
|
| pubmed:abstractText |
We have shown that the second intron of the Podospora mitochondrial gene coding for cytochrome b (Cytb 12) splices autocatalytically, using in vitro transcripts generated from the T7 promoter. The reaction takes place at 37 degrees C in the presence of 50 mM TRIS-HCl pH 7.5, 60 mM MgCl2 and 1 mM GTP but shows a low efficiency even at high KCl concentrations of up to 1.2 M. Under these conditions, intron bI2 follows the conventional pathway of group I splicing, and all characteristic products, with regard to both transesterification and hydrolysis, could be identified. Moreover, the intron is capable of undergoing cyclization, thereby releasing the noncoded G and one additional nucleotide (U) from the 5' end. The 5' cleavage site is preceded by the same two nucleotides, indicating a base-pairing at the same site of the internal guide sequence (IGS) for both splicing and cyclization ("one-binding-site model"). In addition, products resulting from site-specific hydrolysis 138 nucleotides downstream of the 5' splice site were detected. Unusually, the shortened intron is also able to form a circular RNA and an alternative sequence that aligns the cyclization site to the catalytic core of the intron must be assumed.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cytochrome b Group,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Fungal,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Single-Stranded,
http://linkedlifedata.com/resource/pubmed/chemical/Guanosine Triphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/RNA,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Fungal,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, mitochondrial
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
0026-8925
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
233
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
71-80
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:1376408-Ascomycota,
pubmed-meshheading:1376408-Base Sequence,
pubmed-meshheading:1376408-Cytochrome b Group,
pubmed-meshheading:1376408-DNA, Fungal,
pubmed-meshheading:1376408-DNA, Single-Stranded,
pubmed-meshheading:1376408-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:1376408-Guanosine Triphosphate,
pubmed-meshheading:1376408-Introns,
pubmed-meshheading:1376408-Mitochondria,
pubmed-meshheading:1376408-Molecular Sequence Data,
pubmed-meshheading:1376408-Nucleic Acid Hybridization,
pubmed-meshheading:1376408-Plasmids,
pubmed-meshheading:1376408-Promoter Regions, Genetic,
pubmed-meshheading:1376408-RNA,
pubmed-meshheading:1376408-RNA, Fungal,
pubmed-meshheading:1376408-RNA Splicing,
pubmed-meshheading:1376408-Restriction Mapping,
pubmed-meshheading:1376408-Sequence Alignment
|
| pubmed:year |
1992
|
| pubmed:articleTitle |
Self-splicing of a mitochondrial group I intron from the cytochrome b gene of the ascomycete Podospora anserina.
|
| pubmed:affiliation |
Technische Universität Berlin, Fachgebiet Mikrobiologie und Genetik, FRG.
|
| pubmed:publicationType |
Journal Article
|