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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1992-7-13
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pubmed:abstractText |
Capsids of the B19 parvovirus are composed of major (VP2; 58 kD) and minor (VP1; 83 kD) structural proteins. These proteins are identical except for a unique 226 amino acid region at the amino terminus of VP1. Previous immunization studies with recombinant empty capsids have demonstrated that the presence of VP1 was required to elicit virus-neutralizing antibody activity. However, to date, neutralizing epitopes have been identified only on VP2. Crystallographic studies of a related parvovirus (canine parvovirus) suggested the unique amino-terminal portion of VP1 assumed an internal position within the viral capsid. To determine the position of VP1 in both empty capsids and virions, we expressed a fusion protein containing the unique region of VP1. Antisera raised to this protein recognized recombinant empty capsids containing VP1 and VP2, but not those containing VP2 alone, in an enzyme-linked immunosorbent assay. The antisera immunoprecipitated both recombinant empty capsids and human plasma-derived virions, and agglutinated the latter as shown by immune electron microscopy. The sera contained potent neutralizing activity for virus infectivity in vitro. These data indicate that a portion of the amino terminus of VP1 is located on the virion surface, and that this region contains intrinsic neutralizing determinants. The external location of the VP1-specific tail may provide a site for engineered heterologous epitope presentation in novel recombinant vaccines.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1376332,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1376332-1647068,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1376332-1701479,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1376332-1705987,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1376332-1711206,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1376332-1716693,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1376332-1719240,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1376332-2006420,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1376332-2157861,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1376332-2173460,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1376332-2422392,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1376332-2476528,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1376332-2479578,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1376332-2542797,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1376332-2551923,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1376332-2837357,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1376332-2838911,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1376332-2838958,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1376332-3038211,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1376332-3701931,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1376332-3738514
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0021-9738
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
89
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2023-9
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pubmed:dateRevised |
2010-9-7
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pubmed:meshHeading |
pubmed-meshheading:1376332-Animals,
pubmed-meshheading:1376332-Antibodies, Viral,
pubmed-meshheading:1376332-Base Sequence,
pubmed-meshheading:1376332-Capsid,
pubmed-meshheading:1376332-Cell Line,
pubmed-meshheading:1376332-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:1376332-Epitopes,
pubmed-meshheading:1376332-Humans,
pubmed-meshheading:1376332-Microscopy, Electron,
pubmed-meshheading:1376332-Molecular Sequence Data,
pubmed-meshheading:1376332-Oligodeoxyribonucleotides,
pubmed-meshheading:1376332-Parvovirus B19, Human,
pubmed-meshheading:1376332-Polymerase Chain Reaction,
pubmed-meshheading:1376332-Precipitin Tests
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pubmed:year |
1992
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pubmed:articleTitle |
Unique region of the minor capsid protein of human parvovirus B19 is exposed on the virion surface.
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pubmed:affiliation |
Cell Biology Section, National Heart, Lung and Blood Institute, Bethesda, Maryland 20817.
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pubmed:publicationType |
Journal Article
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