Linked Life Data

1376319

Source:http://linkedlifedata.com/resource/pubmed/id/1376319

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
17
pubmed:dateCreated
1992-7-16
pubmed:databankReference
pubmed:abstractText
The cation-dependent mannose 6-phosphate receptor (CD-MPR) is one of the two transmembrane proteins involved in transport of lysosomal enzymes. We have cloned the mouse CD-MPR gene and also a very unusual processed-type CD-MPR pseudogene. They are both present at one copy per haploid genome and map to chromosomes 6 and 3, respectively. Comparison of the complete 10-kilobase (kb) sequence of the functional gene with the cDNA indicates that it contains seven exons. Exon 1 encodes the 5'-untranslated region of the mRNA, the others (exons 2-7) encode the luminal, transmembrane, and cytoplasmic domains of the CD-MPR. Exon 7 also contains a 1.2-kb-long 3'-untranslated region of the mRNA. A unique transcription-initiation site was determined by primer extension of mouse liver mRNA. The promoter elements in the 5' upstream region of this site resemble those contained in genes constitutively transcribed. However, Northern blot analysis demonstrates that the CD-MPR is variably expressed in adult mouse tissues and during mouse development. The pseudogene, which is flanked by direct repeats, is almost colinear with the cDNA indicating that it presumably arose by reverse transcription of an mRNA. However, the pseudogene differs from the cDNA. It contains at its 5' end, an additional 340-nucleotide (nt) sequence homologous to the promoter region of the functional gene. This sequence exhibits some promoter activity in vitro. Furthermore, a 24-nt insertion interrupts the region homologous to the 5'-noncoding region of the cDNA. In the functional gene, this 24-nt sequence occurs between exon 1 and 2, where it is flanked by typical consensus sequences of exon/intron boundaries. Therefore, it may represent an additional exon of the functional gene. These two features of the pseudogene suggest that expression of the CD-MPR gene may be regulated by use of different promoters and/or alternative splicing.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
267
pubmed:geneSymbol
CD-MPR
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
12211-9
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:1376319-Amino Acid Sequence, pubmed-meshheading:1376319-Animals, pubmed-meshheading:1376319-Base Sequence, pubmed-meshheading:1376319-Blotting, Northern, pubmed-meshheading:1376319-Cations, pubmed-meshheading:1376319-Chloramphenicol O-Acetyltransferase, pubmed-meshheading:1376319-Chromosome Mapping, pubmed-meshheading:1376319-DNA, pubmed-meshheading:1376319-Gene Expression, pubmed-meshheading:1376319-Liver, pubmed-meshheading:1376319-Mannosephosphates, pubmed-meshheading:1376319-Mice, pubmed-meshheading:1376319-Molecular Sequence Data, pubmed-meshheading:1376319-Plasmids, pubmed-meshheading:1376319-Promoter Regions, Genetic, pubmed-meshheading:1376319-Pseudogenes, pubmed-meshheading:1376319-RNA, pubmed-meshheading:1376319-RNA Splicing, pubmed-meshheading:1376319-Receptor, IGF Type 2, pubmed-meshheading:1376319-Receptors, Cell Surface, pubmed-meshheading:1376319-Restriction Mapping, pubmed-meshheading:1376319-Sequence Homology, Nucleic Acid, pubmed-meshheading:1376319-Transcription, Genetic
pubmed:year
1992
pubmed:articleTitle
Gene and pseudogene of the mouse cation-dependent mannose 6-phosphate receptor. Genomic organization, expression, and chromosomal localization.
pubmed:affiliation
European Molecular Biology Laboratory, Heidelberg, Federal Republic of Germany.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't