Linked Life Data

1375914

Source:http://linkedlifedata.com/resource/pubmed/id/1375914

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1992-7-7
pubmed:databankReference
pubmed:abstractText
Human endothelial leukocyte-adhesion molecule 1 (ELAM-1), a cell-surface glycoprotein expressed solely on cytokine-activated endothelial cells, mediates the adhesion of blood neutrophils, memory T-cells and some monocytes. ELAM-1, also known as E-selectin or leukocyte endothelial-cell-adhesion molecule 2, is a member of the lectin/epidermal-growth-factor/complement-regulatory-protein-like cell-adhesion molecule family, which includes structurally related molecules referred to as selectins. They are all involved in cell/cell adhesion, playing roles in leukocyte trafficking which are currently only partially defined. We report here the isolation and characterization of the murine equivalent of human ELAM-1. Murine ELAM-1 is encoded by a single-copy gene, spanning about 13 kb, which is structurally organized into 14 exons and 13 introns; very similar to that of its human counterpart. The exon/intron architecture exactly parallels the domain structure of the encoded protein. A murine ELAM-1-specific cDNA was cloned from heart tissue of an interleukin-1-(IL-1)-treated mouse. Its nucleotide sequence shows an overall similarity of 70% to human ELAM-1 cDNA. Transiently expressed in Cos cells, the encoded protein promotes the adhesion between recombinant cells and both human polymorphic nuclear cells, as well as HL60 cells expressing S-Lewis-x sugar moiety. Northern blot studies revealed by far the highest expression of the murine ELAM-1 gene in heart tissue and only low expression in lung tissue of IL-1-treated mice. Within the promoter, most of the recently identified regulatory elements are conserved. An exception is the nuclear factor (NF) kappa B box sequence, which, in the murine ELAM-1 promoter, does not correspond to the consensus NF kappa B sequence (Lenardo and Baltimore, 1989). Band-shift analyses show no binding to NF kappa B-like proteins. However, fusion of the murine ELAM-1 promoter to a chloramphenicol acetyltransferase reporter confers cytokine-inducible transcription, although at a lower level, when compared to the human ELAM-1 promoter. Our results demonstrate the existence of a murine homologue of the human gene and demonstrate for adhesion functional equivalence between the homologous proteins from the two species. In addition, we provide the first evidence of the utility of the murine model in addressing biological questions about the role which ELAM-1 plays in inflammation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0014-2956
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
206
pubmed:geneSymbol
ELAM-1
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
401-11
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:1375914-Amino Acid Sequence, pubmed-meshheading:1375914-Animals, pubmed-meshheading:1375914-Base Sequence, pubmed-meshheading:1375914-Blotting, Northern, pubmed-meshheading:1375914-Cell Adhesion, pubmed-meshheading:1375914-Cell Adhesion Molecules, pubmed-meshheading:1375914-Chloramphenicol O-Acetyltransferase, pubmed-meshheading:1375914-DNA, pubmed-meshheading:1375914-E-Selectin, pubmed-meshheading:1375914-Humans, pubmed-meshheading:1375914-Lung, pubmed-meshheading:1375914-Mice, pubmed-meshheading:1375914-Molecular Sequence Data, pubmed-meshheading:1375914-Myocardium, pubmed-meshheading:1375914-Polymerase Chain Reaction, pubmed-meshheading:1375914-Promoter Regions, Genetic, pubmed-meshheading:1375914-RNA, pubmed-meshheading:1375914-Sequence Homology, Nucleic Acid, pubmed-meshheading:1375914-Transcription, Genetic, pubmed-meshheading:1375914-Transfection
pubmed:year
1992
pubmed:articleTitle
Murine endothelial leukocyte-adhesion molecule 1 is a close structural and functional homologue of the human protein.
pubmed:affiliation
Glaxo Institute for Molecular Biology, Geneva, Switzerland.
pubmed:publicationType
Journal Article