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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1992-6-23
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pubmed:databankReference |
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pubmed:abstractText |
A cDNA clone for the mouse thromboxane A2 receptor was isolated from mouse lung cDNA library. The cDNA has a 1,023 base pair open reading frame which encodes a protein of 341 amino acid residues. STA2 and U-46619 induced inward current in Xenopus laevis oocytes injected with the transcript of the clone. Specific binding of [3H]S-145 was found in membranes of COS-1 cells transfected with the cDNA (Kd = 3.3 nM) and was displaced with unlabeled prostaglandins and thromboxane analogues in the order of S-145 greater than STA2 greater than U-46619 greater than PGD2 greater than PGF2 alpha = PGE2. Northern blot analysis demonstrated that thromboxane A2 receptor mRNA is expressed abundantly in thymus, spleen and lung.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/Oligodeoxyribonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/Poly A,
http://linkedlifedata.com/resource/pubmed/chemical/RNA,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Prostaglandin,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Thromboxane,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Thromboxane A2
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
184
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1197-203
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1375456-Amino Acid Sequence,
pubmed-meshheading:1375456-Animals,
pubmed-meshheading:1375456-Base Sequence,
pubmed-meshheading:1375456-Binding, Competitive,
pubmed-meshheading:1375456-Blotting, Northern,
pubmed-meshheading:1375456-Cell Line,
pubmed-meshheading:1375456-Cell Membrane,
pubmed-meshheading:1375456-Cloning, Molecular,
pubmed-meshheading:1375456-DNA,
pubmed-meshheading:1375456-Gene Library,
pubmed-meshheading:1375456-Humans,
pubmed-meshheading:1375456-Kinetics,
pubmed-meshheading:1375456-Lung,
pubmed-meshheading:1375456-Male,
pubmed-meshheading:1375456-Mice,
pubmed-meshheading:1375456-Molecular Sequence Data,
pubmed-meshheading:1375456-Oligodeoxyribonucleotides,
pubmed-meshheading:1375456-Oocytes,
pubmed-meshheading:1375456-Organ Specificity,
pubmed-meshheading:1375456-Poly A,
pubmed-meshheading:1375456-Polymerase Chain Reaction,
pubmed-meshheading:1375456-RNA,
pubmed-meshheading:1375456-RNA, Messenger,
pubmed-meshheading:1375456-Receptors, Prostaglandin,
pubmed-meshheading:1375456-Receptors, Thromboxane,
pubmed-meshheading:1375456-Recombinant Proteins,
pubmed-meshheading:1375456-Restriction Mapping,
pubmed-meshheading:1375456-Sequence Homology, Nucleic Acid,
pubmed-meshheading:1375456-Thromboxane A2,
pubmed-meshheading:1375456-Transcription, Genetic,
pubmed-meshheading:1375456-Transfection,
pubmed-meshheading:1375456-Xenopus
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pubmed:year |
1992
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pubmed:articleTitle |
Mouse thromboxane A2 receptor: cDNA cloning, expression and northern blot analysis.
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pubmed:affiliation |
Department of Pharmacology, Faculty of Medicine, Kyoto University, Japan.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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