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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
1992-5-28
|
| pubmed:abstractText |
In an effort to identify unique tyrosine kinases found in human leukemia cell lines, we utilized polymerase chain reaction (PCR) technology and degenerate oligonucleotide primers to produce a cDNA library of kinase catalytic domains found in the human monocytic cell line AML-193. This search yielded a member of the class 3 tyrosine kinases closely related to the murine kinase FD-22. Previous work has identified this kinase as JAK1. This class of tyrosine kinases is characterized by being ubiquitously expressed, lacking both a ligand-binding domain and a SH2 domain, while containing a second domain similar to a degenerate kinase domain. Our studies focused on the further characterization of this class 3 tyrosine kinase using Northern blot analysis to demonstrate an increase in steady-state mRNA by interferon-gamma in human monocytes. A human-hamster somatic cell hybrid panel and linkage mapping was used to assign JAK1 (aml-116) to human chromosome 1.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
0950-9232
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
7
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
895-900
|
| pubmed:dateRevised |
2009-11-19
|
| pubmed:meshHeading |
pubmed-meshheading:1373877-Alleles,
pubmed-meshheading:1373877-Amino Acid Sequence,
pubmed-meshheading:1373877-Blotting, Northern,
pubmed-meshheading:1373877-Blotting, Southern,
pubmed-meshheading:1373877-Chromosome Mapping,
pubmed-meshheading:1373877-Chromosomes, Human, Pair 1,
pubmed-meshheading:1373877-Gene Frequency,
pubmed-meshheading:1373877-Gene Library,
pubmed-meshheading:1373877-Humans,
pubmed-meshheading:1373877-Hybrid Cells,
pubmed-meshheading:1373877-Leukemia, Monocytic, Acute,
pubmed-meshheading:1373877-Molecular Sequence Data,
pubmed-meshheading:1373877-Polymerase Chain Reaction,
pubmed-meshheading:1373877-Polymorphism, Restriction Fragment Length,
pubmed-meshheading:1373877-Protein-Tyrosine Kinases,
pubmed-meshheading:1373877-RNA,
pubmed-meshheading:1373877-Sequence Homology, Nucleic Acid,
pubmed-meshheading:1373877-Tumor Cells, Cultured
|
| pubmed:year |
1992
|
| pubmed:articleTitle |
Characterization of a class 3 tyrosine kinase.
|
| pubmed:affiliation |
Biological Carcinogenesis and Development Program, Program Resources, Inc/DynCorp, Frederick, Maryland 21702.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.
|