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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1992-5-26
|
| pubmed:abstractText |
Early work with adenine phosphoribosyltransferase-deficient mutants of CHO cells suggested that a site existed in the third exon of this gene which was preferentially susceptible to mutation by ethyl methanesulphonate. To determine whether this was real we analysed a large collection of induced mutants, and generated a high-density mutational spectrum for this exon. In addition, 4 sites outside exon 3 were analysed by blot. 37 mutations were found in 19 available sites, six of which were at nucleotide 1365, 1 of 2 sites in the putative hotspot (P less than 0.02). One other site, 1308, also was mutated in 6 cell lines and may also be preferentially mutable.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Apr
|
| pubmed:issn |
0027-5107
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
266
|
| pubmed:geneSymbol |
aprt
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
221-30
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:1373832-Adenine Phosphoribosyltransferase,
pubmed-meshheading:1373832-Animals,
pubmed-meshheading:1373832-Base Sequence,
pubmed-meshheading:1373832-CHO Cells,
pubmed-meshheading:1373832-Cricetinae,
pubmed-meshheading:1373832-Molecular Sequence Data,
pubmed-meshheading:1373832-Mutation
|
| pubmed:year |
1992
|
| pubmed:articleTitle |
A mutational hotspot in the aprt gene of Chinese hamster cells.
|
| pubmed:affiliation |
Institut du Cancer de Montréal, Que., Canada.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|