Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1-2
|
| pubmed:dateCreated |
1992-5-19
|
| pubmed:abstractText |
The putative core gene of hepatitis C virus (HCV) was incorporated into a plasmid vector (pCC5-J4), and expressed in Escherichia coli. The product of 180 amino acids (p20c) was purified by gel electrophoresis in the presence of sodium dodecyl sulfate, and used in enzyme-linked immunosorbent assay for antibodies against the putative core protein of HCV (anti-p20c). Anti-p20c was detected in 13 (1.5%) of 873 apparently healthy blood donors. It was detected in 205 (86.5%) of 237 patients with acute or chronic non-A, non-B (NANB) hepatic disease, significantly more frequently (p less than 0.01) than antibodies against the C100-3 protein encoded by nonstructural regions of HCV (anti-C100-3) that was found in 178 (75.1%). Anti-p20c developed in the circulation of a patient with acute NANB hepatitis much earlier than anti-C100-3. HCV RNA was detected by polymerase chain reaction in serum samples from blood donors positive for anti-p20c in high titers, one of which was negative for anti-C100-3. These results indicated that anti-p20c would be useful in complementing anti-C100-3 for the diagnosis of NANB hepatitis and further decreasing the incidence of posttransfusion NANB hepatitis.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Apr
|
| pubmed:issn |
0022-1759
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
8
|
| pubmed:volume |
148
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
N
|
| pubmed:pagination |
143-50
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:1373423-Adolescent,
pubmed-meshheading:1373423-Adult,
pubmed-meshheading:1373423-Blood,
pubmed-meshheading:1373423-Carcinoma, Hepatocellular,
pubmed-meshheading:1373423-Cloning, Molecular,
pubmed-meshheading:1373423-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:1373423-Escherichia coli,
pubmed-meshheading:1373423-Female,
pubmed-meshheading:1373423-Hepacivirus,
pubmed-meshheading:1373423-Hepatitis,
pubmed-meshheading:1373423-Hepatitis C,
pubmed-meshheading:1373423-Humans,
pubmed-meshheading:1373423-Liver Cirrhosis,
pubmed-meshheading:1373423-Liver Neoplasms,
pubmed-meshheading:1373423-Male,
pubmed-meshheading:1373423-Middle Aged,
pubmed-meshheading:1373423-Pilot Projects,
pubmed-meshheading:1373423-Plasmids,
pubmed-meshheading:1373423-Polymerase Chain Reaction,
pubmed-meshheading:1373423-RNA,
pubmed-meshheading:1373423-Viral Core Proteins
|
| pubmed:year |
1992
|
| pubmed:articleTitle |
Serodiagnosis of hepatitis C virus infection by ELISA for antibodies against the putative core protein (p20c) expressed in Escherichia coli.
|
| pubmed:affiliation |
Japanese Red Cross Blood Center, Saitama-Ken.
|
| pubmed:publicationType |
Journal Article,
Comparative Study
|