Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1992-5-5
|
| pubmed:abstractText |
In SPL2-1-2, a murine B-committed immature cell line transformed with a temperature-sensitive mutant of Abelson virus, T cell receptor (TCR) gamma gene rearrangements, together with IgH gene rearrangements, were induced during culture at a non-permissive temperature (39 degrees C). During 11-12 months of culture, TCR gamma gene rearrangements occurred in all cells. In contrast to TCR gamma genes, neither TCR beta or TCR delta were detected even after 11-12 months of culture at a non-permissive temperature. The majority of the TCR gamma gene rearrangements observed here were V gamma 2 to J gamma 2 joinings and the remaining rearrangements were J gamma 1-linked. V gamma 1 to J gamma 4 and V gamma 3 to J gamma 3 joinings were not detected. Approximately 70% of cells with TCR gamma gene rearrangements produced normal-sized transcripts from the rearranged TCR gamma genes. Cloning and sequencing analysis of a cDNA from the transcripts demonstrated that the whole structure of the cDNA was similar to that of T-lineage cells. These results showed that TCR gene rearrangements were restricted to the gamma genes and that V gamma 2 to J gamma 2 joinings occurred preferentially in this B-committed immature cell line. Furthermore, TCR gamma gene rearrangements also occurred in intracytoplasmic mu-chain producing cells. This indicated that the existence of intracytoplasmic mu-chains did not prevent TCR gamma gene rearrangements, although the existence of mu-chains is known to inhibit further productive IgH gene rearrangements, (allelic exclusion). These results should provide many implications for the mechanism of TCR gene rearrangements, especially that of cross-lineage rearrangements.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
0161-5890
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
29
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
401-9
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:1372958-Animals,
pubmed-meshheading:1372958-B-Lymphocytes,
pubmed-meshheading:1372958-Base Sequence,
pubmed-meshheading:1372958-Blotting, Northern,
pubmed-meshheading:1372958-Blotting, Southern,
pubmed-meshheading:1372958-Cell Line,
pubmed-meshheading:1372958-Cloning, Molecular,
pubmed-meshheading:1372958-DNA,
pubmed-meshheading:1372958-Gene Rearrangement, beta-Chain T-Cell Antigen Receptor,
pubmed-meshheading:1372958-Gene Rearrangement, delta-Chain T-Cell Antigen Receptor,
pubmed-meshheading:1372958-Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor,
pubmed-meshheading:1372958-Immunoglobulin Heavy Chains,
pubmed-meshheading:1372958-Mice,
pubmed-meshheading:1372958-Molecular Sequence Data,
pubmed-meshheading:1372958-RNA,
pubmed-meshheading:1372958-Receptors, Antigen, T-Cell, gamma-delta,
pubmed-meshheading:1372958-Restriction Mapping,
pubmed-meshheading:1372958-Sequence Homology, Nucleic Acid,
pubmed-meshheading:1372958-Transformation, Genetic
|
| pubmed:year |
1992
|
| pubmed:articleTitle |
Continuing V gamma 2 to J gamma 2 rearrangements of murine T cell receptor gamma genes in a B-committed immature cell line.
|
| pubmed:affiliation |
Third Department of Internal Medicine, Osaka University Medical School, Japan.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|