Linked Life Data

1372898

Source:http://linkedlifedata.com/resource/pubmed/id/1372898

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
1992-5-4
pubmed:databankReference
pubmed:abstractText
A genomic clone, 536, located at the 44CD region of polytene chromosomes of Drosophila melanogaster, has been characterized for its neurobiological importance. We found that this clone contains a gene which produces 2.6-, 1.3- and 1.1-kilobase (kb) RNAs. While the 2.6-kb RNA is expressed only in the head, the 1.3-kb RNA is present exclusively in the body. The 1.1-kb RNA, however, is found in both the head and body, but in much higher concentration in the head. DNA sequence analysis of a 2.6-kb RNA-specific cDNA showed that this gene encodes a 70-amino acid polypeptide which is the putative Drosophila homologue to the gamma subunit of the bovine G-protein. The Drosophila protein, named D-G gamma 1, shares 46, 43, and 28% identity, and 59, 52, and 60% similarity, with the gamma 2, gamma 3, and gamma t proteins of bovine G proteins, respectively. Sequencing of the 1.1-kb RNA-specific cDNA clone revealed that the 1.1-kb RNA is produced from the 2.6-kb transcription unit by usage of an alternative polyadenylation site, and has a coding region identical to that of the 2.6-kb RNA. Genomic Southern blot hybridization indicated that the Drosophila genome has only one D-G gamma 1 gene. Throughout development the 1.1-kb RNA is found to be the most prevalent species; its level peaks between 9 and 12 h of embryogenesis. As is the case for the other G protein genes of Drosophila, the D-G gamma 1 gene is predominantly expressed in the central nervous system of the fly.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
267
pubmed:geneSymbol
D-G&ggr;1
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6086-92
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:1372898-Amino Acid Sequence, pubmed-meshheading:1372898-Animals, pubmed-meshheading:1372898-Base Sequence, pubmed-meshheading:1372898-Blotting, Northern, pubmed-meshheading:1372898-Cattle, pubmed-meshheading:1372898-Cloning, Molecular, pubmed-meshheading:1372898-DNA, pubmed-meshheading:1372898-Drosophila melanogaster, pubmed-meshheading:1372898-GTP-Binding Proteins, pubmed-meshheading:1372898-Gene Expression, pubmed-meshheading:1372898-Genes, pubmed-meshheading:1372898-Macromolecular Substances, pubmed-meshheading:1372898-Molecular Sequence Data, pubmed-meshheading:1372898-Nervous System Physiological Phenomena, pubmed-meshheading:1372898-Open Reading Frames, pubmed-meshheading:1372898-RNA, pubmed-meshheading:1372898-Restriction Mapping, pubmed-meshheading:1372898-Sequence Homology, Nucleic Acid, pubmed-meshheading:1372898-Software, pubmed-meshheading:1372898-Transcription, Genetic
pubmed:year
1992
pubmed:articleTitle
The Drosophila G protein gamma subunit gene (D-G gamma 1) produces three developmentally regulated transcripts and is predominantly expressed in the central nervous system.
pubmed:affiliation
Department of Zoology, University of Tennessee, Knoxville 37996.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't