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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
1992-5-4
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pubmed:databankReference |
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pubmed:abstractText |
Multidrug resistance of mammalian tumor cells is caused by the enhanced expression of P-glycoproteins. These proteins are encoded by mdr genes and mediate the energy-dependent efflux of a variety of lipophilic drugs from cells. To test whether in plants mdr-like genes might be involved in certain cases of cross-resistance to different herbicides, we have cloned and characterized a gene from Arabidopsis thaliana, atpgp1, encoding a putative P-glycoprotein homologue. Like the mammalian P-glycoproteins, with which it shares extensive sequence homology and a similar organization in structural domains, this protein is internally duplicated. Seven of the nine introns in the atpgp1 gene match introns in the mammalian mdr genes to within a few nucleotides, and the positions of these suggest that P-glycoprotein genes evolved by duplication and subsequent fusion of an intron-containing primordial gene prior to the evolutionary separation of plants and mammals. The atpgp1 gene gives rise to transcripts present in all plant parts but particularly abundant in inflorescence axes.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
25
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pubmed:volume |
267
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pubmed:geneSymbol |
atpgp1,
mdr
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5882-8
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:1372894-Amino Acid Sequence,
pubmed-meshheading:1372894-Base Sequence,
pubmed-meshheading:1372894-Biological Evolution,
pubmed-meshheading:1372894-Blotting, Northern,
pubmed-meshheading:1372894-Blotting, Southern,
pubmed-meshheading:1372894-Cloning, Molecular,
pubmed-meshheading:1372894-DNA,
pubmed-meshheading:1372894-Drug Resistance,
pubmed-meshheading:1372894-Gene Library,
pubmed-meshheading:1372894-Humans,
pubmed-meshheading:1372894-Introns,
pubmed-meshheading:1372894-Membrane Glycoproteins,
pubmed-meshheading:1372894-Molecular Sequence Data,
pubmed-meshheading:1372894-Oligodeoxyribonucleotides,
pubmed-meshheading:1372894-P-Glycoprotein,
pubmed-meshheading:1372894-Plants,
pubmed-meshheading:1372894-Polymerase Chain Reaction,
pubmed-meshheading:1372894-RNA,
pubmed-meshheading:1372894-Sequence Homology, Nucleic Acid,
pubmed-meshheading:1372894-Transcription, Genetic
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pubmed:year |
1992
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pubmed:articleTitle |
Structure of an mdr-like gene from Arabidopsis thaliana. Evolutionary implications.
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pubmed:affiliation |
Institute of Plant Biology, University of Zurich, Switzerland.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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