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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1992-5-4
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pubmed:databankReference |
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pubmed:abstractText |
The characteristics of a cis-acting regulatory region involved in the human-aromatase P-450 gene have been examined by transient expression analysis. The region spans from -242 - -166 relative to the cap site of the gene. A fragment containing the region excised from the gene enhances heterologous promoter activity as well as its own promoter activity in a position-independent and orientation-independent manner. The fragment exerts its enhancer activity in human BeWo choriocarcinoma cells in which the aromatase P-450 gene is expressed, but not in other cell lines tested. Deletion of 38 bp from the 3' end of the fragment results in a complete loss of enhancer activity. A gel-retardation assay with nuclear extracts from BeWo cells suggests the existence of a nuclear factor(s) which interacts with the fragment. These results suggest that the regulatory element in the fragment is involved in efficient transcription of the human-aromatase P-450 gene.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0014-2956
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
205
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
303-9
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:1372865-Animals,
pubmed-meshheading:1372865-Aromatase,
pubmed-meshheading:1372865-Base Sequence,
pubmed-meshheading:1372865-Blotting, Northern,
pubmed-meshheading:1372865-Cell Line,
pubmed-meshheading:1372865-Chloramphenicol O-Acetyltransferase,
pubmed-meshheading:1372865-DNA,
pubmed-meshheading:1372865-Enhancer Elements, Genetic,
pubmed-meshheading:1372865-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:1372865-Humans,
pubmed-meshheading:1372865-Mice,
pubmed-meshheading:1372865-Molecular Sequence Data,
pubmed-meshheading:1372865-Plasmids,
pubmed-meshheading:1372865-Promoter Regions, Genetic,
pubmed-meshheading:1372865-RNA,
pubmed-meshheading:1372865-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:1372865-Transcription, Genetic,
pubmed-meshheading:1372865-Transfection,
pubmed-meshheading:1372865-Tumor Cells, Cultured
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pubmed:year |
1992
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pubmed:articleTitle |
Characterization of a cis-acting regulatory element involved in human-aromatase P-450 gene expression.
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pubmed:affiliation |
Department of Medical Chemistry, Kochi Medical School, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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