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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5052
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pubmed:dateCreated |
1992-4-30
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pubmed:abstractText |
Id is a helix-loop-helix (HLH) protein that represses activity of several basic helix-loop-helix (bHLH) proteins involved in cell type--specific transcription and cell lineage commitment. The myeloid precursor cell line 32DC13(G) expressed Id messenger RNA, which was transiently decreased when cells were induced to terminally differentiate with granulocyte--colony-stimulating factor. Concomitant with the decrease of Id messenger RNA was the appearance in nuclear extracts of DNA binding proteins that recognized a canonical E-box motif, a DNA binding site for some bHLH proteins. Constitutive expression of an Id complementary DNA in 32DC13(G) cells blocked their ability to differentiate and to induce E-box-binding activity. These results suggest that Id and, hence, bHLH proteins function in the process of myeloid differentiation.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0036-8075
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pubmed:author |
|
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pubmed:issnType |
Print
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pubmed:day |
27
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pubmed:volume |
255
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1700-2
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:1372755-Cell Differentiation,
pubmed-meshheading:1372755-Cells, Cultured,
pubmed-meshheading:1372755-DNA-Binding Proteins,
pubmed-meshheading:1372755-Gene Expression,
pubmed-meshheading:1372755-Granulocyte Colony-Stimulating Factor,
pubmed-meshheading:1372755-Hematopoiesis,
pubmed-meshheading:1372755-Inhibitor of Differentiation Protein 1,
pubmed-meshheading:1372755-Interleukin-3,
pubmed-meshheading:1372755-Macromolecular Substances,
pubmed-meshheading:1372755-RNA, Messenger,
pubmed-meshheading:1372755-Repressor Proteins,
pubmed-meshheading:1372755-Transcription Factors
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pubmed:year |
1992
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pubmed:articleTitle |
Inhibition of myeloid differentiation by the helix-loop-helix protein Id.
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pubmed:affiliation |
Wistar Institute of Anatomy and Biology, Philadelphia, PA 19104.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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