Linked Life Data

1372647

Source:http://linkedlifedata.com/resource/pubmed/id/1372647

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1992-4-24
pubmed:abstractText
CD8+ T cell clones specific for a defined epitope present in the circumsporozoite protein of Plasmodium yoelii display striking differences in their in vivo antiplasmodial activity. The adoptive transfer of certain clones (YA23 and YA26) into naive mice inhibits by 90% or more the development of liver stages of malaria parasites and protects against malaria infection. The adoptive transfer of two other T cell clones (YB8 and YA15) results, respectively, in partial or no inhibitory activity on parasite development. We found that "protective" and "nonprotective" cytotoxic T lymphocyte (CTL) clones do not differ in their fine epitope specificity and display similar levels of lysis and DNA degradation of target cells in vitro. Their pattern of production of lymphokines and granule-associated proteins also failed to correlate with their in vivo antiplasmodial activity. Histological studies combined with autoradiography showed that, upon adoptive transfer, only T cells from the protective CTL clones are capable of "associating" with a significant percentage of parasitized hepatocytes. Fluorescence-activated cell sorter analysis of surface molecules revealed pronounced differences in the levels of CD44 and VLA-4 expression by the different clones, correlating closely with their in vivo protective activity. The correlation between in vivo antiparasite activity and the expression of CD44 was further corroborated by the results of sorting, from the partially protective YB8 clone, two sub-populations expressing high and low levels of CD44. These were protective and nonprotective, respectively. The clones also differed in their adhesive properties. Cross-linking of CD44, using specific antibodies, induced LFA-1-mediated homotypic aggregation of protective clones, while nonprotective cells failed to aggregate.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1372647-1694723, http://linkedlifedata.com/resource/pubmed/commentcorrection/1372647-1709674, http://linkedlifedata.com/resource/pubmed/commentcorrection/1372647-1713253, http://linkedlifedata.com/resource/pubmed/commentcorrection/1372647-1714934, http://linkedlifedata.com/resource/pubmed/commentcorrection/1372647-1716146, http://linkedlifedata.com/resource/pubmed/commentcorrection/1372647-1721832, http://linkedlifedata.com/resource/pubmed/commentcorrection/1372647-1919439, http://linkedlifedata.com/resource/pubmed/commentcorrection/1372647-1972715, http://linkedlifedata.com/resource/pubmed/commentcorrection/1372647-1980910, http://linkedlifedata.com/resource/pubmed/commentcorrection/1372647-1993458, http://linkedlifedata.com/resource/pubmed/commentcorrection/1372647-1993837, http://linkedlifedata.com/resource/pubmed/commentcorrection/1372647-2007624, http://linkedlifedata.com/resource/pubmed/commentcorrection/1372647-2142475, http://linkedlifedata.com/resource/pubmed/commentcorrection/1372647-2246503, http://linkedlifedata.com/resource/pubmed/commentcorrection/1372647-2440339, http://linkedlifedata.com/resource/pubmed/commentcorrection/1372647-2442176, http://linkedlifedata.com/resource/pubmed/commentcorrection/1372647-2477703, http://linkedlifedata.com/resource/pubmed/commentcorrection/1372647-2527858, http://linkedlifedata.com/resource/pubmed/commentcorrection/1372647-2695102, http://linkedlifedata.com/resource/pubmed/commentcorrection/1372647-3085218, http://linkedlifedata.com/resource/pubmed/commentcorrection/1372647-6609997
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0022-1007
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
175
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
895-905
pubmed:dateRevised
2010-9-7
pubmed:meshHeading
pubmed-meshheading:1372647-Amino Acid Sequence, pubmed-meshheading:1372647-Animals, pubmed-meshheading:1372647-Antigens, CD8, pubmed-meshheading:1372647-Antigens, Protozoan, pubmed-meshheading:1372647-Cell Adhesion, pubmed-meshheading:1372647-Cell Adhesion Molecules, pubmed-meshheading:1372647-Clone Cells, pubmed-meshheading:1372647-Cytotoxicity, Immunologic, pubmed-meshheading:1372647-Epitopes, pubmed-meshheading:1372647-Immunization, Passive, pubmed-meshheading:1372647-Intercellular Adhesion Molecule-1, pubmed-meshheading:1372647-Liver, pubmed-meshheading:1372647-Lymphocyte Function-Associated Antigen-1, pubmed-meshheading:1372647-Mice, pubmed-meshheading:1372647-Mice, Inbred Strains, pubmed-meshheading:1372647-Molecular Sequence Data, pubmed-meshheading:1372647-Plasmodium yoelii, pubmed-meshheading:1372647-Receptors, Lymphocyte Homing, pubmed-meshheading:1372647-Receptors, Very Late Antigen, pubmed-meshheading:1372647-T-Lymphocytes, Cytotoxic
pubmed:year
1992
pubmed:articleTitle
The in vivo cytotoxic activity of CD8+ T cell clones correlates with their levels of expression of adhesion molecules.
pubmed:affiliation
Department of Medical and Molecular Parasitology, New York University School of Medicine, New York 10010.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't