|
rdf:type |
|
|
lifeskim:mentions |
umls-concept:C0003250,
umls-concept:C0003320,
umls-concept:C0037224,
umls-concept:C0205208,
umls-concept:C0332256,
umls-concept:C0441513,
umls-concept:C0598086,
umls-concept:C0598388,
umls-concept:C1415887,
umls-concept:C1419040,
umls-concept:C1420433,
umls-concept:C1424666,
umls-concept:C1527177,
umls-concept:C1704241
|
|
pubmed:dateCreated |
1992-4-16
|
|
pubmed:abstractText |
We have previously shown that immunization with solid matrix-antigen-antibody (SMAA) complexes induces both vigorous humoral and cell-mediated immune responses and have suggested that this method of vaccination may be developed for use in humans, and potentially as a vaccine against AIDS. Here we demonstrate that a small oligopeptide can act as a tag for the construction of SMAA complexes using a tag-specific monoclonal antibody and tag-linked antigens. We show that a 14-amino acid oligopeptide, present in the phospho (P) and V proteins of simian virus 5 (SV5), retains its antigenicity when attached to the C terminus of three 'foreign' proteins [p27 and gp110 of simian immunodeficiency virus (SIV) and glutathione S-transferase] such that these proteins can be incorporated into SMAA complexes using a monoclonal antibody (MAb) that was originally raised against the native SV5 P and V proteins. Mice were immunized with SMAA complexes containing recombinant p27-TAG and MAbs have been isolated that recognized native SIV p27. The significance of these results in terms of the development of SMAA complexes as human vaccines is discussed.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigen-Antibody Complex,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes,
http://linkedlifedata.com/resource/pubmed/chemical/Gag protein p27, Simian...,
http://linkedlifedata.com/resource/pubmed/chemical/Gene Products, gag,
http://linkedlifedata.com/resource/pubmed/chemical/HIV Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/Oligopeptides,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Vaccines, Synthetic,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Envelope Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/gp 110 env protein, Human...
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Mar
|
|
pubmed:issn |
0022-1317
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
73 ( Pt 3)
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
653-60
|
|
pubmed:dateRevised |
2006-11-15
|
|
pubmed:meshHeading |
pubmed-meshheading:1372038-Amino Acid Sequence,
pubmed-meshheading:1372038-Animals,
pubmed-meshheading:1372038-Antibodies, Monoclonal,
pubmed-meshheading:1372038-Antigen-Antibody Complex,
pubmed-meshheading:1372038-Antigens, Viral,
pubmed-meshheading:1372038-Base Sequence,
pubmed-meshheading:1372038-Epitopes,
pubmed-meshheading:1372038-Gene Products, gag,
pubmed-meshheading:1372038-Genetic Vectors,
pubmed-meshheading:1372038-HIV Antigens,
pubmed-meshheading:1372038-Mice,
pubmed-meshheading:1372038-Molecular Sequence Data,
pubmed-meshheading:1372038-Oligopeptides,
pubmed-meshheading:1372038-Paramyxoviridae,
pubmed-meshheading:1372038-Recombinant Proteins,
pubmed-meshheading:1372038-Simian immunodeficiency virus,
pubmed-meshheading:1372038-Vaccines, Synthetic,
pubmed-meshheading:1372038-Viral Envelope Proteins
|
|
pubmed:year |
1992
|
|
pubmed:articleTitle |
Construction of solid matrix-antibody-antigen complexes containing simian immunodeficiency virus p27 using tag-specific monoclonal antibody and tag-linked antigen.
|
|
pubmed:affiliation |
Department of Biochemistry and Microbiology, University of St Andrews, Fife, U.K.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
