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rdf:type |
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lifeskim:mentions |
umls-concept:C0001455,
umls-concept:C0017067,
umls-concept:C0021852,
umls-concept:C0030956,
umls-concept:C0439849,
umls-concept:C0441889,
umls-concept:C0445223,
umls-concept:C0999699,
umls-concept:C1280500,
umls-concept:C1552599,
umls-concept:C1704787
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pubmed:issue |
1
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pubmed:dateCreated |
1992-4-8
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pubmed:abstractText |
This study was designed to test the hypothesis that stimulation of adenylate cyclase and elevation of cAMP is involved in the signal transduction process for substance P, calcitonin gene-related peptide, vasoactive intestinal peptide, cholecystokinin or gastrin releasing peptide in myenteric ganglia. Enzymatically dissociated ganglia from the myenteric plexus of the guinea-pig small intestine were used to study changes in levels of cAMP in response to application of the brain-gut peptides in the presence and absence of forskolin. Application of substance P and calcitonin gene-related peptide were found to increase intraganglionic cAMP in a dose-dependent fashion when a phosphodiesterase inhibitor was present. The ED50 values for substance P and calcitonin gene-related peptide were 5 microM and 0.75 microM, respectively. The presence of forskolin in the incubation medium resulted in significant upward shifts of the dose-response curves for both peptides. Neither vasoactive intestinal peptide, cholecystokinin nor gastrin releasing peptide stimulated increases in intraganglionic cAMP under the same experimental conditions used for substance P and calcitonin gene-related peptide.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0014-2999
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
14
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pubmed:volume |
225
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
21-7
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:1371754-1-Methyl-3-isobutylxanthine,
pubmed-meshheading:1371754-4-(3-Butoxy-4-methoxybenzyl)-2-imidazolidinone,
pubmed-meshheading:1371754-Animals,
pubmed-meshheading:1371754-Calcitonin Gene-Related Peptide,
pubmed-meshheading:1371754-Cholecystokinin,
pubmed-meshheading:1371754-Cyclic AMP,
pubmed-meshheading:1371754-Dose-Response Relationship, Drug,
pubmed-meshheading:1371754-Ganglia,
pubmed-meshheading:1371754-Gastrin-Releasing Peptide,
pubmed-meshheading:1371754-Guinea Pigs,
pubmed-meshheading:1371754-Intestine, Small,
pubmed-meshheading:1371754-Male,
pubmed-meshheading:1371754-Myenteric Plexus,
pubmed-meshheading:1371754-Peptides,
pubmed-meshheading:1371754-Signal Transduction,
pubmed-meshheading:1371754-Substance P,
pubmed-meshheading:1371754-Vasoactive Intestinal Peptide
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pubmed:year |
1992
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pubmed:articleTitle |
Effects of brain-gut related peptides on cAMP levels in myenteric ganglia of guinea-pig small intestine.
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pubmed:affiliation |
Department of Physiology, College of Medicine, Ohio State University, Columbus 43210.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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