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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1992-3-24
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pubmed:abstractText |
The mode of activation of an H(+)-conducting pathway present in the membrane of neutrophils was investigated. (1) Resting neutrophils released protons through an electrogenic Cd(2+)-inhibitable (K0.5 approximately 20 microM) route when a pH gradient and appropriate charge compensation was provided. (2) The rate of H+ efflux was stimulated over 2.5-fold by 4 beta-phorbol 12-myristate 13-acetate (PMA; K0.5 approximately 0.7 nM) or by 4 beta-phorbol 12,13-dibutyrate (K0.5 approximately 20 nM) even when the NADPH oxidase was blocked by p-chloromercuribenzoate. (3) Staurosporine inhibited the effect of PMA. (4) The H+ egress was not enhanced by 4 alpha-phorbol 12,13-didecanoate. (5) Low concentrations of Cd2+ (less than 40 microM) inhibited the H+ flux without influencing the oxidase. The results raise the possibility that protein kinase C could be involved in the activation of an electrogenic H(+)-conducting pathway in the membrane of neutrophils. The activation of this route by phorbol esters seems to be independent of the stimulation of NADPH oxidase.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1371386-2139663,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1371386-2145772,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1371386-2147429,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1371386-2155923,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1371386-2166029,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1371386-2166041,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1371386-2171344,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1371386-2172230,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1371386-2174779,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1371386-2425631,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1371386-2435712,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1371386-2456757,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1371386-2481858,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1371386-2526587,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1371386-2539191,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1371386-2546827,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1371386-2559721,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1371386-2579574,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1371386-2656760,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1371386-2825632,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1371386-2831954,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1371386-2843166,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1371386-2848318,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1371386-2848319,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1371386-2848506,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1371386-2904439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1371386-2985050,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1371386-2997160,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1371386-3021215,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1371386-3030326,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1371386-3800872,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1371386-3821511,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1371386-6249851,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1371386-6311812,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1371386-6490651,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1371386-6865930,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1371386-6953983,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1371386-7159582
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cadmium,
http://linkedlifedata.com/resource/pubmed/chemical/Chloromercuribenzoates,
http://linkedlifedata.com/resource/pubmed/chemical/Ion Channels,
http://linkedlifedata.com/resource/pubmed/chemical/NADH, NADPH Oxidoreductases,
http://linkedlifedata.com/resource/pubmed/chemical/NADPH Oxidase,
http://linkedlifedata.com/resource/pubmed/chemical/Phorbol 12,13-Dibutyrate,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C,
http://linkedlifedata.com/resource/pubmed/chemical/Protons,
http://linkedlifedata.com/resource/pubmed/chemical/Tetradecanoylphorbol Acetate,
http://linkedlifedata.com/resource/pubmed/chemical/Valinomycin,
http://linkedlifedata.com/resource/pubmed/chemical/p-Chloromercuribenzoic Acid
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
281 ( Pt 3)
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
697-701
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pubmed:dateRevised |
2010-9-7
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pubmed:meshHeading |
pubmed-meshheading:1371386-Animals,
pubmed-meshheading:1371386-Biological Transport,
pubmed-meshheading:1371386-Cadmium,
pubmed-meshheading:1371386-Cell Membrane,
pubmed-meshheading:1371386-Cell Membrane Permeability,
pubmed-meshheading:1371386-Chloromercuribenzoates,
pubmed-meshheading:1371386-Hydrogen-Ion Concentration,
pubmed-meshheading:1371386-Ion Channels,
pubmed-meshheading:1371386-NADH, NADPH Oxidoreductases,
pubmed-meshheading:1371386-NADPH Oxidase,
pubmed-meshheading:1371386-Neutrophils,
pubmed-meshheading:1371386-Phorbol 12,13-Dibutyrate,
pubmed-meshheading:1371386-Protein Kinase C,
pubmed-meshheading:1371386-Protons,
pubmed-meshheading:1371386-Swine,
pubmed-meshheading:1371386-Tetradecanoylphorbol Acetate,
pubmed-meshheading:1371386-Valinomycin,
pubmed-meshheading:1371386-p-Chloromercuribenzoic Acid
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pubmed:year |
1992
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pubmed:articleTitle |
Phorbol 12-myristate 13-acetate activates an electrogenic H(+)-conducting pathway in the membrane of neutrophils.
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pubmed:affiliation |
Department of Physiology, Semmelweis University Medical School, Budapest, Hungary.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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