Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1992-3-26
pubmed:abstractText
We present a novel method for the PCR amplification of unknown DNA that flanks a known segment directly from human genomic DNA. PCR requires that primer annealing sites be present on each end of the DNA segment that is to be amplified. In this method, known DNA is placed on the uncharacterized side of the sequence of interest via DNA polymerase mediated generation of a PCR template that is shaped like a pan with a handle. Generation of this template permits specific amplification of the unknown sequence. Taq (DNA) polymerase was used to form the original template and to generate the PCR product. 2.2 kb of the beta-globin gene, and 657 bp of the 5' flanking region of the cystic fibrosis transmembrane conductance regulator gene, were amplified directly from human genomic DNA using primers that initially flank only one side of the region amplified. This method will provide a powerful tool for acquiring DNA sequence information.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1371352-1709163, http://linkedlifedata.com/resource/pubmed/commentcorrection/1371352-1710598, http://linkedlifedata.com/resource/pubmed/commentcorrection/1371352-2003926, http://linkedlifedata.com/resource/pubmed/commentcorrection/1371352-2161516, http://linkedlifedata.com/resource/pubmed/commentcorrection/1371352-2183192, http://linkedlifedata.com/resource/pubmed/commentcorrection/1371352-2190192, http://linkedlifedata.com/resource/pubmed/commentcorrection/1371352-2191081, http://linkedlifedata.com/resource/pubmed/commentcorrection/1371352-2349121, http://linkedlifedata.com/resource/pubmed/commentcorrection/1371352-2349129, http://linkedlifedata.com/resource/pubmed/commentcorrection/1371352-2448875, http://linkedlifedata.com/resource/pubmed/commentcorrection/1371352-2461560, http://linkedlifedata.com/resource/pubmed/commentcorrection/1371352-2463672, http://linkedlifedata.com/resource/pubmed/commentcorrection/1371352-2475911, http://linkedlifedata.com/resource/pubmed/commentcorrection/1371352-2704070, http://linkedlifedata.com/resource/pubmed/commentcorrection/1371352-2788276, http://linkedlifedata.com/resource/pubmed/commentcorrection/1371352-2814500, http://linkedlifedata.com/resource/pubmed/commentcorrection/1371352-2814502, http://linkedlifedata.com/resource/pubmed/commentcorrection/1371352-2852134, http://linkedlifedata.com/resource/pubmed/commentcorrection/1371352-2999980, http://linkedlifedata.com/resource/pubmed/commentcorrection/1371352-3033825, http://linkedlifedata.com/resource/pubmed/commentcorrection/1371352-3047679, http://linkedlifedata.com/resource/pubmed/commentcorrection/1371352-3157994, http://linkedlifedata.com/resource/pubmed/commentcorrection/1371352-3472723, http://linkedlifedata.com/resource/pubmed/commentcorrection/1371352-6093122, http://linkedlifedata.com/resource/pubmed/commentcorrection/1371352-6199744, http://linkedlifedata.com/resource/pubmed/commentcorrection/1371352-6254664
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0305-1048
pubmed:author
pubmed:issnType
Print
pubmed:day
11
pubmed:volume
20
pubmed:geneSymbol
CFTR
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
595-600
pubmed:dateRevised
2010-9-7
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Sequence specific generation of a DNA panhandle permits PCR amplification of unknown flanking DNA.
pubmed:affiliation
Department of Pediatrics, University of Iowa College of Medicine, Iowa City 52242.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't