Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1992-2-20
pubmed:abstractText
P-selectin (PADGEM, GMP-140, CD62) is a transmembrane protein specific to alpha granules of platelets and Weibel-Palade bodies of endotheial cells. Upon stimulation of these cells, P-selectin is translocated to the plasma membrane where it functions as a receptor for monocytes and neutrophils. To investigate whether the mechanism of targeting of P-selectin to granules is specific for megakaryocytes and endothelial cells and/or dependent on von Willebrand factor, a soluble adhesive protein that is stored in the same granules, we have expressed the cDNA for P-selectin in AtT-20 cells. AtT-20 cells are a mouse pituitary cell line that can store proteins in a regulated fashion. By double-label immunofluorescence, P-selectin was visible as a punctate pattern at the tips of cell processes. This pattern closely resembled the localization of ACTH, the endogenous hormone produced and stored by the AtT-20 cells. Fractionation of the transfected cells resulted in the codistribution of P-selectin and ACTH in cellular compartments of the same density. Immunoelectron microscopy using a polyclonal anti-P-selectin antibody demonstrated immunogold localization in dense granules, morphologically indistinguishable from the ACTH granules. Binding experiments with radiolabeled monoclonal antibody to P-selectin indicated that there was also surface expression of P-selectin on the AtT-20 cells. After stimulation with the secretagogue 8-Bromo-cAMP the surface expression increased twofold, concomitant with the release of ACTH. In contrast, the surface expression of P-selectin transfected into CHO cells, which do not have a regulated pathway of secretion, did not change with 8-Br-cAMP treatment. In conclusion, we provide evidence for the regulated secretion of a transmembrane protein (P-selectin) in a heterologous cell line, which indicates that P-selectin contains an independent sorting signal directing it to storage granules.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1370497-14228505, http://linkedlifedata.com/resource/pubmed/commentcorrection/1370497-14444755, http://linkedlifedata.com/resource/pubmed/commentcorrection/1370497-1688717, http://linkedlifedata.com/resource/pubmed/commentcorrection/1370497-1689464, http://linkedlifedata.com/resource/pubmed/commentcorrection/1370497-1699666, http://linkedlifedata.com/resource/pubmed/commentcorrection/1370497-1717161, http://linkedlifedata.com/resource/pubmed/commentcorrection/1370497-1847928, http://linkedlifedata.com/resource/pubmed/commentcorrection/1370497-1974032, http://linkedlifedata.com/resource/pubmed/commentcorrection/1370497-1987472, http://linkedlifedata.com/resource/pubmed/commentcorrection/1370497-1988154, http://linkedlifedata.com/resource/pubmed/commentcorrection/1370497-2040652, http://linkedlifedata.com/resource/pubmed/commentcorrection/1370497-2110571, http://linkedlifedata.com/resource/pubmed/commentcorrection/1370497-2180925, http://linkedlifedata.com/resource/pubmed/commentcorrection/1370497-2209556, http://linkedlifedata.com/resource/pubmed/commentcorrection/1370497-2307706, http://linkedlifedata.com/resource/pubmed/commentcorrection/1370497-2391371, http://linkedlifedata.com/resource/pubmed/commentcorrection/1370497-2411738, http://linkedlifedata.com/resource/pubmed/commentcorrection/1370497-2443536, http://linkedlifedata.com/resource/pubmed/commentcorrection/1370497-2463989, http://linkedlifedata.com/resource/pubmed/commentcorrection/1370497-2466335, http://linkedlifedata.com/resource/pubmed/commentcorrection/1370497-2466574, http://linkedlifedata.com/resource/pubmed/commentcorrection/1370497-2467701, http://linkedlifedata.com/resource/pubmed/commentcorrection/1370497-2470733, http://linkedlifedata.com/resource/pubmed/commentcorrection/1370497-2472431, http://linkedlifedata.com/resource/pubmed/commentcorrection/1370497-2478294, http://linkedlifedata.com/resource/pubmed/commentcorrection/1370497-2545730, http://linkedlifedata.com/resource/pubmed/commentcorrection/1370497-2551036, http://linkedlifedata.com/resource/pubmed/commentcorrection/1370497-2564811, http://linkedlifedata.com/resource/pubmed/commentcorrection/1370497-2565905, http://linkedlifedata.com/resource/pubmed/commentcorrection/1370497-2657740, http://linkedlifedata.com/resource/pubmed/commentcorrection/1370497-2663878, http://linkedlifedata.com/resource/pubmed/commentcorrection/1370497-2745426, http://linkedlifedata.com/resource/pubmed/commentcorrection/1370497-2826013, http://linkedlifedata.com/resource/pubmed/commentcorrection/1370497-2827173, http://linkedlifedata.com/resource/pubmed/commentcorrection/1370497-2828380, http://linkedlifedata.com/resource/pubmed/commentcorrection/1370497-2838491, http://linkedlifedata.com/resource/pubmed/commentcorrection/1370497-2842866, http://linkedlifedata.com/resource/pubmed/commentcorrection/1370497-2941452, http://linkedlifedata.com/resource/pubmed/commentcorrection/1370497-3017997, http://linkedlifedata.com/resource/pubmed/commentcorrection/1370497-3031082, http://linkedlifedata.com/resource/pubmed/commentcorrection/1370497-3040770, http://linkedlifedata.com/resource/pubmed/commentcorrection/1370497-3082891, http://linkedlifedata.com/resource/pubmed/commentcorrection/1370497-3092864, http://linkedlifedata.com/resource/pubmed/commentcorrection/1370497-3318877, http://linkedlifedata.com/resource/pubmed/commentcorrection/1370497-3494734, http://linkedlifedata.com/resource/pubmed/commentcorrection/1370497-3877532, http://linkedlifedata.com/resource/pubmed/commentcorrection/1370497-5129551, http://linkedlifedata.com/resource/pubmed/commentcorrection/1370497-5432063, http://linkedlifedata.com/resource/pubmed/commentcorrection/1370497-6264438, http://linkedlifedata.com/resource/pubmed/commentcorrection/1370497-6292032, http://linkedlifedata.com/resource/pubmed/commentcorrection/1370497-6300682, http://linkedlifedata.com/resource/pubmed/commentcorrection/1370497-6317196, http://linkedlifedata.com/resource/pubmed/commentcorrection/1370497-6754744
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0021-9525
pubmed:author
pubmed:issnType
Print
pubmed:volume
116
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
617-25
pubmed:dateRevised
2010-9-7
pubmed:meshHeading
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