Linked Life Data

1369422

Source:http://linkedlifedata.com/resource/pubmed/id/1369422

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1993-3-25
pubmed:abstractText
We have used the pressure dependency of density in near critical propane to design a novel protein extraction system. Active, structurally intact, proteins have been extracted from an aqueous phase into subcritical propane containing the non-ionic detergent polyoxyethylene sorbitan trioleate (Tween-85). The pressure dependence of protein transfer from the aqueous to organic phase is described for cytochrome C and subtilisin. The effect of the microemulsion and compressible propane on protein structure and function is also discussed. The first determinations of kinetic constants for an enzyme-catalyzed reaction in such a system are also presented.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
B
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0733-222X
pubmed:author
pubmed:issnType
Print
pubmed:volume
10
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1584-8
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Solubilization and activity of proteins in compressible-fluid based microemulsions.
pubmed:affiliation
Department of Chemical Engineering, University of Pittsburgh, Pennsylvania 15261.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't