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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
8
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pubmed:dateCreated |
1992-11-6
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pubmed:abstractText |
Acidocin 8912, a bacteriocin produced by Lactobacillus acidophilus TK8912, was purified by ammonium sulfate fractionation and successive chromatographies on CM-cellulose, Sephadex G-50, Sephadex G-25, and reversed-phase HPLC on Aquapore RP-300. The purified acidocin 8912 migrated as a single band on SDS-PAGE. The molecular weight was estimated to be 5200 by SDS-PAGE, and 5400 by HPLC gel filtration on TSKgel G3000PWXL. Both the amino acid composition and the N-terminal amino acid sequence analysis indicated that acidocin 8912 was a peptide composed of presumably 50 amino acids containing a Lys residue at the N-terminus. The purified acidocin 8912 showed a bactericidal effect on sensitive cells but not a bacteriolytic effect.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
B
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0916-8451
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
56
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1212-5
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pubmed:dateRevised |
2000-12-18
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pubmed:meshHeading |
pubmed-meshheading:1368836-Amino Acid Sequence,
pubmed-meshheading:1368836-Amino Acids,
pubmed-meshheading:1368836-Ammonium Sulfate,
pubmed-meshheading:1368836-Bacteriocins,
pubmed-meshheading:1368836-Chromatography, High Pressure Liquid,
pubmed-meshheading:1368836-Lactobacillus acidophilus,
pubmed-meshheading:1368836-Microbial Sensitivity Tests,
pubmed-meshheading:1368836-Molecular Sequence Data,
pubmed-meshheading:1368836-Molecular Weight
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pubmed:year |
1992
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pubmed:articleTitle |
Purification and some properties of acidocin 8912, a novel bacteriocin produced by Lactobacillus acidophilus TK8912.
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pubmed:affiliation |
Research Laboratory, Tamon Sake Brewing Co., Ltd., Hyogo, Japan.
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pubmed:publicationType |
Journal Article
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