Linked Life Data

1368817

Source:http://linkedlifedata.com/resource/pubmed/id/1368817

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1992-11-17
pubmed:abstractText
As most high density and immobilized fermentation systems do not allow the direct quantitative determination of cell density, two flow cytometric methods (the determination of incorporation of bromodeoxyuridine into newly synthesized DNA and the increase in mitotic cells by colchicine blockage) were evaluated as to their suitability to measure true division rates of cells in bioreactors. The BrdU method gave division rates identical to the growth rates measured by cell count, while the colchicine block method gave values that were lower and varied with the cell line. This is due to the cytotoxicity of colchicine and makes a calibration of the method for each cell line necessary. Both methods have been successfully used to measure division rates of rCHO cells immobilized in an alginate matrix as well as in macroporous carriers in a fluidised bed system and in dialysis culture.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
B
pubmed:status
MEDLINE
pubmed:issn
0920-9069
pubmed:author
pubmed:issnType
Print
pubmed:volume
8
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
207-14
pubmed:dateRevised
2008-10-9
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Determination of division rates of rCHO cells in high density and immobilized fermentation systems by flow cytometry.
pubmed:affiliation
Institute of Applied Microbiology, University of Agriculture, Vienna, Austria.
pubmed:publicationType
Journal Article