rdf:type |
|
lifeskim:mentions |
|
pubmed:issue |
11
|
pubmed:dateCreated |
1992-3-10
|
pubmed:abstractText |
We rapidly and efficiently isolated the 5'-region of cDNA encoding the N-terminal region of human centromere antigen B (CENP-B) including an ATG methionine codon by polymerase chain reactions (PCR). The unknown 5'-flanking sequence of the cDNA was amplified using an adaptor-sequence ligated to the 5' end as a universal primer sequence. To locate the target fragments, we did an additional PCR with another set of two internal primers using samples of the size-fractionated products as templates, rather than using the conventional hybridization procedure. This approach can further be applied to the analysis of other unknown flanking sequences of cDNA or genomic DNA.
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
B
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:month |
Nov
|
pubmed:issn |
0002-1369
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:volume |
55
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
2687-92
|
pubmed:dateRevised |
2006-11-15
|
pubmed:meshHeading |
pubmed-meshheading:1368745-Autoantigens,
pubmed-meshheading:1368745-Base Sequence,
pubmed-meshheading:1368745-Centromere Protein B,
pubmed-meshheading:1368745-Chromosomal Proteins, Non-Histone,
pubmed-meshheading:1368745-Cloning, Molecular,
pubmed-meshheading:1368745-DNA,
pubmed-meshheading:1368745-DNA-Binding Proteins,
pubmed-meshheading:1368745-Gene Amplification,
pubmed-meshheading:1368745-Genome, Human,
pubmed-meshheading:1368745-Humans,
pubmed-meshheading:1368745-Molecular Sequence Data,
pubmed-meshheading:1368745-Polymerase Chain Reaction,
pubmed-meshheading:1368745-Templates, Genetic
|
pubmed:year |
1991
|
pubmed:articleTitle |
A rapid isolation of the unknown 5'-flanking sequence of human CENP-B cDNA with polymerase chain reactions.
|
pubmed:affiliation |
Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture, Japan.
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|