Linked Life Data

1368682

Source:http://linkedlifedata.com/resource/pubmed/id/1368682

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1991-8-12
pubmed:abstractText
A cDNA library corresponding to mite protein was screened using anti-Der f II, a major allergen from the house dust mite Dermatophagoides farinae, antibody. Three possible clones were obtained that contained cDNA fragments coding for Der f II, and the nucleotide sequences of the fragments were determined. There were minor differences observed affecting the deduced amino acid sequence among the three cDNA fragments. The amino acid sequence of the purified native Der f II protein could be analyzed to 45 residues from the N-terminus. As a result of comparison, all the three cDNA fragments code for a mature protein with a derived molecular weight of about 14,000. The amino acid sequence was not homologous to any known protein sequences and it contained six cysteine residues and no N-glycosylation sites.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
B
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0002-1369
pubmed:author
pubmed:issnType
Print
pubmed:volume
55
pubmed:geneSymbol
Der f II
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1233-8
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
Cloning and expression of cDNA coding for the major house dust mite allergen Der f II in Escherichia coli.
pubmed:affiliation
Central Research Laboratories, Asahi Breweries, Ltd., Tokyo, Japan.
pubmed:publicationType
Journal Article