Linked Life Data

1368396

Source:http://linkedlifedata.com/resource/pubmed/id/1368396

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1992-9-17
pubmed:abstractText
During I.13.17. hybridoma batch cultivation, the levels of cytoplasmic mRNAs specifying immunoglobulin heavy (H) and light (L) chains peaked during the exponential phase and decreased significantly during the decline phase. This variation was found to parallel the rate of DNA synthesis and the total cytoplasmic RNA content. Moreover, we have investigated the possibility that the stability of H- and L-chain mRNAs could account for the observed difference in their levels throughout the batch culture. Using a transcription inhibitor (actinomycin D), we found that the differential accumulation of both mRNAs reflected transcript stability. However, at any growth stage of the culture, the specific monoclonal antibody production rate (pg IgG cell-1 h-1) was not reflected by the levels of H- or L-chain mRNAs, but could be partially correlated to the specific metabolic activity of the cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
B
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0141-0229
pubmed:author
pubmed:issnType
Print
pubmed:volume
14
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
135-40
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Kinetic studies of cellular metabolic activity, specific IgG production rate, IgG mRNA stability and accumulation during hybridoma batch culture.
pubmed:affiliation
Institut Pasteur, Département des Biotechnologies, Paris, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't