Linked Life Data

13678340

Source:http://linkedlifedata.com/resource/pubmed/id/13678340

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2003-9-18
pubmed:abstractText
Immediately after X-irradiation, monocytic cells can express the gene for interleukin (IL)-1beta, which enhances inflammation and contributes to radioprotection in mice. In order to analyze the mechanism(s) for the immediate-early induction of IL-1beta after X-irradiation at 20 Gy in cultured murine macrophages, we examined the molecules that bound to the DNA fragments corresponding to the upstream region of 10 kb of the mouse IL-1beta gene using an electrophoretic mobility-shift assay. Three DNA fragments corresponding to the 8,500, 8,000 and 2,500 bases upstream of the gene showed an unique binding site with the nuclear extract. Specific binding activity with these DNA fragments was observed in the nuclear extract from non-irradiated cells, and disappeared upon a pretreatment of the extract with proteinase K. The binding activity was not detected in the nuclear extract from irradiated cells. This shows that protein(s) specifically binding to the far-upstream regions of the IL-1beta gene disappear immediately after X-irradiation in the nuclei of macrophage cells, and that the event is potentially related to the immediate-early response of IL-1beta gene expression.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0449-3060
pubmed:author
pubmed:issnType
Print
pubmed:volume
44
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
117-23
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Disappearance of nuclear binding proteins specifically bound to the upstream region of the interleukin-1beta gene immediately after irradiation of mouse macrophages.
pubmed:affiliation
Redox Regulation Research Group, National Institute of Radiological Sciences, 9-1 Anagawa 4-Chome, Inage-ku, Chiba-shi, Chiba 263-8555, Japan. ishihara@nirs.go.jp
pubmed:publicationType
Journal Article