|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
7
|
|
pubmed:dateCreated |
1990-12-14
|
|
pubmed:abstractText |
We have employed the baculovirus expression system for the production of a mouse monoclonal IgG antibody directed against lipoprotein I of Pseudomonas aeruginosa. Both light and heavy chain cDNAs were introduced into the baculovirus genome in a single step of homologous recombination. Insect cells that were infected with the recombinant virus stably secreted antigen-binding and glycosylated antibody molecules capable of binding the complement component C1q.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
B
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jul
|
|
pubmed:issn |
0733-222X
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
8
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
651-4
|
|
pubmed:dateRevised |
2006-4-21
|
|
pubmed:meshHeading |
pubmed-meshheading:1367456-Animals,
pubmed-meshheading:1367456-Antibodies, Bacterial,
pubmed-meshheading:1367456-Antibodies, Monoclonal,
pubmed-meshheading:1367456-Baculoviridae,
pubmed-meshheading:1367456-Cloning, Molecular,
pubmed-meshheading:1367456-Complement C1,
pubmed-meshheading:1367456-Genetic Vectors,
pubmed-meshheading:1367456-Glycosylation,
pubmed-meshheading:1367456-Immunoglobulin G,
pubmed-meshheading:1367456-Immunoglobulin Heavy Chains,
pubmed-meshheading:1367456-Immunoglobulin Light Chains,
pubmed-meshheading:1367456-Insects,
pubmed-meshheading:1367456-Lipoproteins,
pubmed-meshheading:1367456-Mice,
pubmed-meshheading:1367456-Pseudomonas aeruginosa,
pubmed-meshheading:1367456-Recombinant Proteins
|
|
pubmed:year |
1990
|
|
pubmed:articleTitle |
Antibody production in baculovirus-infected insect cells.
|
|
pubmed:affiliation |
Laboratorium für molekulare Biologie-Genzentrum-der Ludwig-Maximilians-Universität München, Martinsried, FRG.
|
|
pubmed:publicationType |
Journal Article
|
