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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1990-8-27
|
| pubmed:abstractText |
We report the construction and use of a new system for the direct screening of recombinant clones after transformation. The system uses a Bacillus subtilis-Escherichia coli shuttle vector that carries the B. subtilis structural gene for alpha-amylase. Insertion of foreign DNA into this gene results in a loss of amylolytic activity in the host cells that can be assayed using a simple and inexpensive staining procedure.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
B
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
0733-222X
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
8
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
241-2
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:1367421-Bacillus subtilis,
pubmed-meshheading:1367421-Chromosome Mapping,
pubmed-meshheading:1367421-Cloning, Molecular,
pubmed-meshheading:1367421-DNA, Recombinant,
pubmed-meshheading:1367421-Escherichia coli,
pubmed-meshheading:1367421-Genetic Markers,
pubmed-meshheading:1367421-Genetic Vectors,
pubmed-meshheading:1367421-Plasmids,
pubmed-meshheading:1367421-Transformation, Bacterial,
pubmed-meshheading:1367421-alpha-Amylases
|
| pubmed:year |
1990
|
| pubmed:articleTitle |
The alpha-amylase gene as a marker for gene cloning: direct screening of recombinant clones.
|
| pubmed:affiliation |
Genetics Institute, Escola Superior de Agricultura Luiz de Queiroz, Universidade de Sao Paulo, Brazil.
|
| pubmed:publicationType |
Journal Article
|