Linked Life Data

1366929

Source:http://linkedlifedata.com/resource/pubmed/id/1366929

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1991-4-30
pubmed:abstractText
We replaced the wild-type TOX operon of Bordetella pertussis with in vitro mutated, detoxified alleles by electroporetic transformation using unmarked linear DNA. Uptake of DNA was selected by transient ampicillin resistance and two simultaneous recombination events resulted in gene-replacement at the natural locus with no integration of heterologous DNA. TOX alleles were stable without selection and recombinant strains secreted non-toxic, fully assembled, protective pertussis toxin (PT) analogues with kinetics similar to the parental vaccine strain under production-scale fermentation conditions. Strains generated in this way are suitable for the production of recombinant whole-cell or component whooping cough vaccines that require no chemical modification of PT.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
B
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0733-222X
pubmed:author
pubmed:issnType
Print
pubmed:volume
8
pubmed:geneSymbol
S1
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1025-9
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Gene replacement in Bordetella pertussis by transformation with linear DNA.
pubmed:affiliation
Connaught Centre for Biotechnology Research, Willowdale, Ontario, Canada.
pubmed:publicationType
Journal Article