Linked Life Data

1365468

Source:http://linkedlifedata.com/resource/pubmed/id/1365468

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1995-2-2
pubmed:abstractText
A new in vitro model for studying cartilage breakdown has been utilised in this work. Polymorphonuclear neutrophils (PMNs) with phorbol myristate acetate (PMA) were layered onto 2 microns cryostat sections of bovine nasal cartilage. After incubation, the sections were fixed, stained, and the amount of glycosaminoglycan (GAG) contents measured by microdensitometry. PMNs caused GAG loss from sections and this was greatly enhanced when the PMNs were activated by PMA. Various pharmacological agents were then added to the system, namely acetyl salicylic acid, indomethacin, ibuprofen, piroxicam, dexamethasone, D-penicillamine, chloroquine and BN50548. The drugs tested had no direct effect on cartilage matrix, nor did they affect GAG loss from sections treated with non-stimulated PMNs. However, BN50548, a novel protease inhibitor, afforded a dose response protection of cartilage section from GAG loss by PMA stimulated PMNs. This model may prove to be of value in screening novel antiproteases with chondroprotective activity.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0140-1610
pubmed:author
pubmed:issnType
Print
pubmed:volume
11
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
19-22
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
Drug effects on a novel in vitro model of cartilage breakdown.
pubmed:affiliation
Department of Experimental Pathology, St. Bartholomew's Hospital Medical College, London, U.K.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't