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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1995-1-5
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pubmed:abstractText |
1. Perivascular nerves of the sheep middle cerebral artery show immunoreactivity for both vasoactive intestinal polypeptide (VIP) and calcitonin gene-related peptide (CGRP). 2. Rings of endothelium-denuded sheep middle cerebral artery precontracted with 5-hydroxytryptamine were relaxed by CGRP (maximum relaxation = 87.8 +/- 8.1%, pD2 = 7.81 +/- 0.12, n = 12) and by VIP (maximum relaxation = 55.1 +/- 4.1%, pD2 = 7.65 +/- 0.04, n = 18). Rings of endothelium-denuded cat middle cerebral artery precontracted with U46619 were also relaxed by vasoactive intestinal polypeptide (maximum relaxation = 53.1 +/- 6.1%, pD2 = 7.82 +/- 0.11, n = 6). 3. Haemolysate (1 microliters ml-1) inhibited VIP-induced relaxation in endothelium-denuded sheep and cat middle cerebral artery (n = 6) but had no effect on the CGRP-induced relaxation of the sheep middle cerebral artery (n = 6). 4. The relaxant response to VIP in endothelium-denuded sheep middle cerebral artery was inhibited by methylene blue (10 microM) and augmented by either M&B 22948 (10 microM) or superoxide dismutase (150 units ml-1). Indomethacin (1 microM) had no effect. 5. The addition of L-NG-monomethyl arginine (100 microM) inhibited both neurogenic and VIP-induced relaxation of endothelium-denuded sheep MCA by 56 +/- 6% and 60 +/- 6% (n = 5) respectively. The CGRP-induced relaxation was unaffected. 6. It is concluded that neurally mediated vasodilatation in the sheep middle cerebral artery is mediated largely by VIP through a direct action on smooth muscle through a cyclic-GMP-mediated mechanism that appears to involve synthesis of nitric oxide from L-arginine. Vasodilatation by CGRP, which is also contained in perivascular nerves, does not utilize this pathway.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1364820-1970342,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1364820-2430233,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1364820-2432665,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1364820-2442009,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1364820-2448322,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1364820-2501869,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1364820-2581921,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1364820-2611482,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1364820-2612562,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1364820-27076,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1364820-2783499,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1364820-2783612,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1364820-2983068,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1364820-2993964,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1364820-2995876,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1364820-3390182,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1364820-34490,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1364820-3500957,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1364820-3886679,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1364820-4396591,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1364820-5063989,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1364820-6112057,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1364820-6182281,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1364820-6206080,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1364820-6316142,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1364820-6358944,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1364820-6360964,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1364820-6382196,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1364820-6396532,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1364820-6470062,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1364820-6719122,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1364820-6771016
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0007-1188
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
102
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
567-72
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pubmed:dateRevised |
2010-9-7
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pubmed:meshHeading |
pubmed-meshheading:1364820-Animals,
pubmed-meshheading:1364820-Arginine,
pubmed-meshheading:1364820-Calcitonin Gene-Related Peptide,
pubmed-meshheading:1364820-Cerebral Arteries,
pubmed-meshheading:1364820-Electric Stimulation,
pubmed-meshheading:1364820-Endothelium, Vascular,
pubmed-meshheading:1364820-Immunohistochemistry,
pubmed-meshheading:1364820-Nitric Oxide,
pubmed-meshheading:1364820-Sheep,
pubmed-meshheading:1364820-Vasoactive Intestinal Peptide,
pubmed-meshheading:1364820-Vasodilation,
pubmed-meshheading:1364820-omega-N-Methylarginine
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pubmed:year |
1991
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pubmed:articleTitle |
Relaxation of sheep cerebral arteries by vasoactive intestinal polypeptide and neurogenic stimulation: inhibition by L-NG-monomethyl arginine in endothelium-denuded vessels.
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pubmed:affiliation |
Department of Physiology & Pharmacology, University of Strathclyde, Glasgow.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
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