Linked Life Data

1364033

Source:http://linkedlifedata.com/resource/pubmed/id/1364033

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1993-9-16
pubmed:abstractText
Possible involvement of microtubules in the regulation of granulosa cell steroidogenesis by large follicle granulosa cell conditioned media (LGCCM) was assessed by monitoring the effect of agents that alter the cytoplasmic microtubule-tubulin equilibrium. The changes in microtubule organization and cell shape were examined by immunohistochemical procedure and morphometric analysis. Progesterone production stimulated by LGCCM was reduced by colchicine (agent that polymerizes microtubules) in a dose-dependent manner. In contrast, LGCCM stimulated progesterone secretion was significantly decreased by microtubule stabilizing agent (taxol: 1-10 microM). Cultured granulosa cells with a flattened appearance and projections after 24 h of incubation assumed a spherical configuration and were devoid of cytoplasmic processes when cultured with these agents. LGCCM stimulated GCs showed a reduction in the perimeter as compared with controls, although some cytoplasmic processes were observed. These findings suggest the involvement of microtubules in the regulation of cultured granulosa cell progesterone production by LGCCM, possibly through an effect on subcellular organelle distribution by altering the morphology of granulosa cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1210-0668
pubmed:author
pubmed:issnType
Print
pubmed:volume
26
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
195-9
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Microtubules and regulation of granulosa cell steroidogenesis by porcine granulosa cell conditioned medium.
pubmed:affiliation
Institute of Cell Biology and Morphology, Bulgarian Academy of Sciences, Sofia.
pubmed:publicationType
Journal Article