Linked Life Data

1361732

Source:http://linkedlifedata.com/resource/pubmed/id/1361732

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1993-1-28
pubmed:abstractText
P-glycoprotein (P-gp), the multidrug resistance gene product, is overexpressed in normal adult rat hepatocytes under standard culture conditions. We have studied the modulation of P-gp expression in this in vitro model in the presence of both epidermal growth factor and pyruvate, which favor hepatocyte growth, as well as in the presence of either dimethyl sulfoxide (DMSO) or nicotinamide, which favors maintenance of differentiated functions. P-gp overexpression, estimated by northern blotting and doxorubicin-mediated drug efflux analyses, was similarly observed during culture in both standard and proliferating conditions, while it was delayed, but not inhibited, in the presence of DMSO or nicotinamide. These results suggest that the functional P-gp overexpression occurring in rat hepatocytes when exposed to an unfamiliar environment is at least partly not related to cell proliferation or the degree of cell differentiation in vitro.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0006-2952
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
44
pubmed:geneSymbol
mdr
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2259-62
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Modulation of multidrug resistance gene expression in rat hepatocytes maintained under various culture conditions.
pubmed:affiliation
Unité de Recherches, Hépatologiques, U 49 de l'INSERM, Hôpital de Pontchaillou, Rennes, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't