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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
6
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pubmed:dateCreated |
1993-1-27
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pubmed:abstractText |
The study of human microvascular endothelial cells has been limited, because these cells are difficult to isolate in pure culture, are fastidious in their in vitro growth requirements, and have a very limited lifespan. In order to overcome these difficulties, we have transfected human dermal microvascular endothelial cells (HMEC) with a PBR-322-based plasmid containing the coding region for the simian virus 40 A gene product, large T antigen, and succeeded in immortalizing them. These cells, termed CDC/EU.HMEC-1 (HMEC-1), have been passaged 95 times to date and show no signs of senescence, whereas normal microvascular endothelial cells undergo senescence at passages 8-10. HMEC-1 exhibit typical cobblestone morphology when grown in monolayer culture, express and secrete von Willebrand's Factor, take up acteylated low-density lipoprotein, and rapidly form tubes when cultured on matrigel. HMEC-1 grow to densities three to seven times higher than microvascular endothelial cells and require much less stringent growth medium. HMEC-1 will grow in the absence of human serum, whereas microvascular endothelial cells require culture medium supplemented with 30% human serum. These cells express other cell-surface molecules typically associated with endothelial cells, including CD31 and CD36 and epitopes identified by monoclonal antibodies EN4 and PAL-E. They also express the cell adhesion molecules ICAM-1 and CD44 and following stimulation with interferon-gamma express major histocompatibility complex class II antigens. HMEC-1 specifically bind lymphocytes in cell adhesion assays. Thus HMEC-1 is the first immortalized human microvascular endothelial cell line that retains the morphologic, phenotypic, and functional characteristics of normal human microvascular endothelial cells.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD31,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation...,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Polyomavirus Transforming,
http://linkedlifedata.com/resource/pubmed/chemical/Cell Adhesion Molecules,
http://linkedlifedata.com/resource/pubmed/chemical/Intercellular Adhesion Molecule-1
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0022-202X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
99
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
683-90
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pubmed:dateRevised |
2004-11-17
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pubmed:meshHeading |
pubmed-meshheading:1361507-Antigens, CD31,
pubmed-meshheading:1361507-Antigens, Differentiation, Myelomonocytic,
pubmed-meshheading:1361507-Antigens, Polyomavirus Transforming,
pubmed-meshheading:1361507-Cell Adhesion,
pubmed-meshheading:1361507-Cell Adhesion Molecules,
pubmed-meshheading:1361507-Cell Line,
pubmed-meshheading:1361507-Endothelium, Vascular,
pubmed-meshheading:1361507-Humans,
pubmed-meshheading:1361507-Intercellular Adhesion Molecule-1,
pubmed-meshheading:1361507-Male,
pubmed-meshheading:1361507-Microcirculation,
pubmed-meshheading:1361507-Phenotype,
pubmed-meshheading:1361507-T-Lymphocytes,
pubmed-meshheading:1361507-Transfection
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pubmed:year |
1992
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pubmed:articleTitle |
HMEC-1: establishment of an immortalized human microvascular endothelial cell line.
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pubmed:affiliation |
Biological Products Branch, Centers for Disease Control, Atlanta, Georgia.
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pubmed:publicationType |
Journal Article
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