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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
24
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pubmed:dateCreated |
1993-1-19
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pubmed:abstractText |
Monoclonal antibody 2F10 is an "internal-image" anti-idiotype (anti-id) antibody capable of mimicking the group-specific "a" determinant of human hepatitis B surface antigen (HBsAg). By mRNA sequencing and computer-assisted molecular modeling of monoclonal antibody 2F10, we identified a 15-amino acid region of the heavy-chain hypervariable region that has partial residue homology with sequences of the "a" determinant epitopes of HBsAg. We have established that a linear 15-mer peptide from a contiguous region on the anti-id antibody can (i) generate anti-HBsAg-specific antibodies when injected into mice, (ii) prime murine lymph node cells for in vitro HBsAg-specific T-cell proliferative responses, and (iii) stimulate in vitro human CD4+ T cells that were primed in vivo to HBsAg by natural infection with hepatitis B virus or vaccination with a commercially available HBsAg vaccine. Significantly, this peptide could also stimulate CD4+ T cells of human hepatitis B virus carriers. We conclude that a 15-mer peptide derived from the anti-id sequence can duplicate the B- and T-cell stimulatory activity of the intact anti-id antibody and the antigen that is mimicked, HBsAg.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1361231-1079326,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1361231-1384823,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1361231-15036,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1361231-1568715,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1361231-1697396,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1361231-1724616,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1361231-2040800,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1361231-2408999,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1361231-2428036,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1361231-2455014,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1361231-2457914,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1361231-2687698,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1361231-2693610,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1361231-2786032,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1361231-3053890,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1361231-3113826,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1361231-3286496,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1361231-3419534,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1361231-351618,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1361231-3681981,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1361231-388439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1361231-3937730,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1361231-399329,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1361231-4142565,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1361231-471053,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1361231-493148,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1361231-5420325,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1361231-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1361231-5551392,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1361231-6173154,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1361231-6181506,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1361231-6187828,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1361231-6197187,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1361231-6204768,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1361231-6320100,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1361231-6337208,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1361231-6755247
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
89
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
11900-4
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pubmed:dateRevised |
2010-9-7
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pubmed:meshHeading |
pubmed-meshheading:1361231-Amino Acid Sequence,
pubmed-meshheading:1361231-Animals,
pubmed-meshheading:1361231-Antibodies, Anti-Idiotypic,
pubmed-meshheading:1361231-Base Sequence,
pubmed-meshheading:1361231-CD4-Positive T-Lymphocytes,
pubmed-meshheading:1361231-Hepatitis B Surface Antigens,
pubmed-meshheading:1361231-Humans,
pubmed-meshheading:1361231-Lymphocyte Activation,
pubmed-meshheading:1361231-Mice,
pubmed-meshheading:1361231-Mice, Inbred BALB C,
pubmed-meshheading:1361231-Molecular Sequence Data,
pubmed-meshheading:1361231-Oligodeoxyribonucleotides,
pubmed-meshheading:1361231-Peptides
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pubmed:year |
1992
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pubmed:articleTitle |
Molecular mimicry of hepatitis B surface antigen by an anti-idiotype-derived synthetic peptide.
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pubmed:affiliation |
Department of Molecular Immunology, Roswell Park Cancer Institute, Buffalo, NY 14263.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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