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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1-3
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pubmed:dateCreated |
1993-1-8
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pubmed:abstractText |
The major heat shock proteins from Thiobacillus ferrooxidans were identified as DnaK and GroEL equivalents by Western blotting and analysis of the N-terminal amino acid sequence of spots isolated from dried 2-D polyacrylamide electrophoresis gels. The T. ferrooxidans chaperonins showed 70% and 80% identity with the Escherichia coli GroEL and DnaK, respectively. By using electrophoresis with a transverse pore gradient of cross-linked polyacrylamide and nondenaturing conditions followed by Western blotting, we found that the GroEL proteins from both bacteria formed a 14-mer, whereas E. coli DnaK protein existed partially as a dimer and the T. ferrooxidans DnaK-equivalent showed only a monomeric nature under our experimental conditions.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0378-1097
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
77
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
149-53
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:1360930-Amino Acid Sequence,
pubmed-meshheading:1360930-Bacterial Proteins,
pubmed-meshheading:1360930-Blotting, Western,
pubmed-meshheading:1360930-Chaperonin 60,
pubmed-meshheading:1360930-Escherichia coli,
pubmed-meshheading:1360930-Escherichia coli Proteins,
pubmed-meshheading:1360930-HSP70 Heat-Shock Proteins,
pubmed-meshheading:1360930-Heat-Shock Proteins,
pubmed-meshheading:1360930-Molecular Sequence Data,
pubmed-meshheading:1360930-Protein Conformation,
pubmed-meshheading:1360930-Sequence Homology, Amino Acid,
pubmed-meshheading:1360930-Species Specificity,
pubmed-meshheading:1360930-Thiobacillus
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pubmed:year |
1992
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pubmed:articleTitle |
Identification and characterization of GroEL and DnaK homologues in Thiobacillus ferrooxidans.
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pubmed:affiliation |
Departamento de Bioquímica, Facultad de Medicina, Universidad de Chile, Santiago.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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