Linked Life Data

13598812

Source:http://linkedlifedata.com/resource/pubmed/id/13598812

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1959-12-1
pubmed:abstractText
When one or two drops of a dilute, non-irritating solution of 2,4-dinitrochlorobenzene (DNCB) is applied to a small area of skin of the intact guinea pig, about 20 per cent of the applied material, or some derivative of it, is soon excreted in urine. In normal, as well as in specifically sensitized guinea pigs, DNCB at the site of local application becomes rapidly bound to skin protein through primary chemical bonds. Twenty-four hours after application roughly half of the material present at the local skin site is still extractable with organic solvents. Of the non-extractable dinitrophenyl groups, about 99 per cent are in epidermis, and about 85 per cent are substituted in epsilon-NH(2) groups of lysine residues. Only traces of bound dinitrophenyl groups were observed in the corium. It is uncertain whether these are formed in situ, or are experimental contaminants, or are migratory epidermally formed conjugates. Even when DNCB is injected intradermally it combines predominantly with overlying epidermis and with epidermal components of hair follicles, but only slightly with corium. The 2,4-dinitrophenyl conjugates which are localized in the deeper, viable half of the epidermis, close to the epidermal-dermal junction, are inferred to be the agents responsible for specifically evoking the allergic response in sensitized animals. Conjugates which are situated in the outer, cornified half of the epidermis are shown to be incapable of eliciting the allergic response. The results furnish a basis for interpreting a common pattern of lesions in allergic contact dermatitis as it occurs spontaneously in man.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/13598812-13069525, http://linkedlifedata.com/resource/pubmed/commentcorrection/13598812-13212051, http://linkedlifedata.com/resource/pubmed/commentcorrection/13598812-13293468, http://linkedlifedata.com/resource/pubmed/commentcorrection/13598812-13385403, http://linkedlifedata.com/resource/pubmed/commentcorrection/13598812-13459877, http://linkedlifedata.com/resource/pubmed/commentcorrection/13598812-13512852, http://linkedlifedata.com/resource/pubmed/commentcorrection/13598812-14904452, http://linkedlifedata.com/resource/pubmed/commentcorrection/13598812-14927800, http://linkedlifedata.com/resource/pubmed/commentcorrection/13598812-15442895, http://linkedlifedata.com/resource/pubmed/commentcorrection/13598812-16748281, http://linkedlifedata.com/resource/pubmed/commentcorrection/13598812-18152339, http://linkedlifedata.com/resource/pubmed/commentcorrection/13598812-19870383, http://linkedlifedata.com/resource/pubmed/commentcorrection/13598812-19970041
pubmed:keyword
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
OM
pubmed:chemical
pubmed:status
OLDMEDLINE
pubmed:month
Dec
pubmed:issn
0022-1007
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
108
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
773-96
pubmed:dateRevised
2010-9-21
pubmed:meshHeading
pubmed:year
1958
pubmed:articleTitle
Elicitation of allergic contact dermatitis in the guinea pig; the distribution of bound dinitrobenzene groups within the skin and quantitative determination of the extent of combination of 2,4-dinitro-chlorobenzene with epidermal protein in vivo.
pubmed:publicationType
Journal Article