Linked Life Data

1358028

Source:http://linkedlifedata.com/resource/pubmed/id/1358028

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1992-11-13
pubmed:abstractText
Twenty-four species belonging to the genus Pichia were investigated using restriction fragment length polymorphism (RFLP) and Southern blot hybridization of their genomic DNA. Saccharomyces cerevisiae, Kluyveromyces lactis, Williopsis mrakii and Candida albicans were also included in this study. The RFLP patterns were obtained from digestion of yeast DNA with several restriction endonuclease enzymes, and showed various bands with different mobility; in most isolates, the more deeply stained bands were species-specific. This observation was confirmed by the results obtained from Southern blot hybridization of the EcoRI and XhoI RFLP patterns with P. anomala UCSC 25F DNA, digested with the same enzymes, used as probes. These bands are likely to be ribosomal DNA as shown by hybridization of digested DNA from unrelated yeast species (S. cerevisiae, K. lactis and C. albicans). However, one hybridized band, located at 3.9-4.1 Kb, seems to be peculiar to the Pichia species. Our study confirms the usefulness of molecular tools in studying genetic relatedness among yeasts.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0003-6072
pubmed:author
pubmed:issnType
Print
pubmed:volume
62
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
215-23
pubmed:dateRevised
2008-8-22
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Genomic studies on killer yeasts belonging to the genus Pichia.
pubmed:affiliation
Istituto di Microbiologia, Università Cattolica del Sacro Cuore, Roma, Italy.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't