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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1977-1-3
|
| pubmed:abstractText |
The generation of mouse cytolytic T lymphocytes (CTL) in primary or secondary mixed leukocyte cultures (MLC) is greatly enhanced by the addition of 2-mercaptoethanol (2-ME) to the culture medium. This enhancement can be equally demonstrated with either reduced or oxidized 2-ME. Addition of 2-ME to MLC as late as 3 days after the initiation of the culture results in a peak CTL response which is nearly as high as the one in cultures containing 2-ME throughout the 4 day-incubation period. Quantitative analysis of the CTL response in MLC supplemented with 2-ME on day 3 shows a 50-fold increase in lytic activity within 24 h, suggesting that cell differentiation, in addition to proliferation may be affected by this agent.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
0014-2980
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
5
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
223-5
|
| pubmed:dateRevised |
2003-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:135683-Animals,
pubmed-meshheading:135683-Cytotoxicity Tests, Immunologic,
pubmed-meshheading:135683-Female,
pubmed-meshheading:135683-Immunity, Cellular,
pubmed-meshheading:135683-Lymphocyte Culture Test, Mixed,
pubmed-meshheading:135683-Mercaptoethanol,
pubmed-meshheading:135683-Mice,
pubmed-meshheading:135683-Mice, Inbred Strains,
pubmed-meshheading:135683-Oxidation-Reduction,
pubmed-meshheading:135683-T-Lymphocytes,
pubmed-meshheading:135683-Time Factors
|
| pubmed:year |
1975
|
| pubmed:articleTitle |
Effect of delayed addition of 2-mercaptoethanol on the generation of mouse cytotoxic T lymphocytes in mixed leukocyte cultures.
|
| pubmed:publicationType |
Journal Article
|