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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1992-7-10
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pubmed:abstractText |
We previously demonstrated that combined treatment of mice with crude oil and longwave ultraviolet radiation (UVA) led to the depletion of IA-positive cells from the epidermis. In the present study, we have developed an in vitro screening assay for combined effects of purified petrochemicals and UVA on epidermal IA and Thy-1 expression. This method involves removal of skin from donor mice prior to treatment with chemicals and UVA (20,000 J/m2), followed by in vitro culture and subsequent immunoperoxidase staining. In this study, a complete correlation was observed in terms of IA-positive cell density among similarly treated cultured skin and live mice. In vivo and in vitro studies both indicated that anthracene but not phenanthrene or benzo[a]pyrene led to significant depletion of both epidermal Langerhans cells and Thy-1-positive dendritic cells when followed by UVA treatment. The in vitro assay developed for this study should prove to be a valuable tool for the screening of a wide variety of chemicals for contact photosensitizing activity.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Anthracenes,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Thy-1,
http://linkedlifedata.com/resource/pubmed/chemical/Benzo(a)pyrene,
http://linkedlifedata.com/resource/pubmed/chemical/Histocompatibility Antigens Class II,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Petroleum,
http://linkedlifedata.com/resource/pubmed/chemical/Phenanthrenes,
http://linkedlifedata.com/resource/pubmed/chemical/Polycyclic Compounds,
http://linkedlifedata.com/resource/pubmed/chemical/anthracene,
http://linkedlifedata.com/resource/pubmed/chemical/phenanthrene
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0098-4108
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
35
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
175-85
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1351103-Animals,
pubmed-meshheading:1351103-Anthracenes,
pubmed-meshheading:1351103-Antigens, Surface,
pubmed-meshheading:1351103-Antigens, Thy-1,
pubmed-meshheading:1351103-Benzo(a)pyrene,
pubmed-meshheading:1351103-Cell Count,
pubmed-meshheading:1351103-Culture Techniques,
pubmed-meshheading:1351103-Dendritic Cells,
pubmed-meshheading:1351103-Dose-Response Relationship, Drug,
pubmed-meshheading:1351103-Dose-Response Relationship, Radiation,
pubmed-meshheading:1351103-Epidermis,
pubmed-meshheading:1351103-Female,
pubmed-meshheading:1351103-Histocompatibility Antigens Class II,
pubmed-meshheading:1351103-Immunoenzyme Techniques,
pubmed-meshheading:1351103-Langerhans Cells,
pubmed-meshheading:1351103-Membrane Glycoproteins,
pubmed-meshheading:1351103-Mice,
pubmed-meshheading:1351103-Mice, Inbred C3H,
pubmed-meshheading:1351103-Petroleum,
pubmed-meshheading:1351103-Phenanthrenes,
pubmed-meshheading:1351103-Polycyclic Compounds,
pubmed-meshheading:1351103-Ultraviolet Rays
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pubmed:year |
1992
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pubmed:articleTitle |
Effects of petrochemicals and ultraviolet radiation on epidermal IA expression in vitro.
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pubmed:affiliation |
Department of Microbiology, Oklahoma State University, Stillwater 74078.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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