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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1992-7-14
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pubmed:abstractText |
Previous studies using anti-CD45R monoclonal antibodies (mAb) have shown that normal CD4+ T cells can be separated into virgin and memory cells based on their level of expression of CD45R. CD45Rhi (virgin) T cells secrete interleukin (IL)-2 whereas CD45Rlo (memory) T cells secrete both IL-2 and IL-4. In contrast to these results, studies using cultured T cell lines have shown that IL-2-secreting T helper cell type 1 (Th1) cells are CD45Rlo and IL-4-secreting Th2 cells are CD45Rhi. To resolve this discrepancy, and to determine how the Th1 and Th2 cell lines relate to memory CD45Rlo and virgin CD45Rhi CD4+ T cells, we have isolated CD45Rlo cells from alloantigen-primed mice and developed allospecific Th1 and Th2 cell lines. The lymphokines secreted by these lines were then evaluated. Our results show that as CD45Rlo T cells develop into in vitro cell lines which secrete IL-4, they become CD45Rhi. In contrast, CD45Rlo cells that develop into lines which secrete IL-2 maintain their CD45Rlo phenotype. Thus, although both Th1 and Th2 cells arise initially from CD45Rlo cells, Th2 cells become CD45Rhi during prolonged in vitro culture but, Th1 cells do not.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD3,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD45,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation...,
http://linkedlifedata.com/resource/pubmed/chemical/Concanavalin A,
http://linkedlifedata.com/resource/pubmed/chemical/Histocompatibility Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-2,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-4,
http://linkedlifedata.com/resource/pubmed/chemical/Ionomycin,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Tyrosine Phosphatase...,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell,
http://linkedlifedata.com/resource/pubmed/chemical/Tetradecanoylphorbol Acetate
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0014-2980
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
22
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1455-9
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:1350982-Animals,
pubmed-meshheading:1350982-Antigens, CD,
pubmed-meshheading:1350982-Antigens, CD3,
pubmed-meshheading:1350982-Antigens, CD45,
pubmed-meshheading:1350982-Antigens, Differentiation, T-Lymphocyte,
pubmed-meshheading:1350982-CD4-Positive T-Lymphocytes,
pubmed-meshheading:1350982-Cell Line,
pubmed-meshheading:1350982-Cells, Cultured,
pubmed-meshheading:1350982-Concanavalin A,
pubmed-meshheading:1350982-Flow Cytometry,
pubmed-meshheading:1350982-Gene Expression,
pubmed-meshheading:1350982-Histocompatibility Antigens,
pubmed-meshheading:1350982-Interferon-gamma,
pubmed-meshheading:1350982-Interleukin-2,
pubmed-meshheading:1350982-Interleukin-4,
pubmed-meshheading:1350982-Ionomycin,
pubmed-meshheading:1350982-Mice,
pubmed-meshheading:1350982-Mice, Inbred BALB C,
pubmed-meshheading:1350982-Mice, Inbred C57BL,
pubmed-meshheading:1350982-Protein Tyrosine Phosphatase, Non-Receptor Type 1,
pubmed-meshheading:1350982-Receptors, Antigen, T-Cell,
pubmed-meshheading:1350982-Tetradecanoylphorbol Acetate
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pubmed:year |
1992
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pubmed:articleTitle |
Changes in expression of CD45R during the development of Th1 and Th2 cell lines.
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pubmed:affiliation |
Department of Microbiology, University of Texas Southwestern Medical Center, Dallas 75235.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.
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