Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1992-5-15
pubmed:abstractText
Treatment of SH-SY5Y human neuroblastoma cells with the protein kinase inhibitor staurosporine, induced both morphological and functional differentiation in these cells. The effects of staurosporine were comparable to those induced by the protein kinase C (PKC) activator, 12-O-tetradecanoyl phorbol 13-acetate (TPA), with respect to induction of neuronal differentiation, i.e. neurite outgrowth, inhibition of DNA synthesis, induction and down-regulation of c-myc protein expression, induction of mRNA for both neuropeptide Y (NPY) and growth associated protein 43 (GAP-43) and stimulation of tyrosine hydroxylase expression. Staurosporine failed to translocate PKC to the membrane fraction or to stimulate phosphorylation of the endogenous PKC substrate M(r) 80,000 (p80). Instead, staurosporine inhibited TPA-induced phosphorylation of p80.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0014-5793
pubmed:author
pubmed:issnType
Print
pubmed:day
30
pubmed:volume
300
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
114-8
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:1348695-Alkaloids, pubmed-meshheading:1348695-Blotting, Northern, pubmed-meshheading:1348695-Blotting, Western, pubmed-meshheading:1348695-Cell Differentiation, pubmed-meshheading:1348695-GAP-43 Protein, pubmed-meshheading:1348695-Gene Expression Regulation, pubmed-meshheading:1348695-Humans, pubmed-meshheading:1348695-Membrane Glycoproteins, pubmed-meshheading:1348695-Nerve Tissue Proteins, pubmed-meshheading:1348695-Neurons, pubmed-meshheading:1348695-Neuropeptide Y, pubmed-meshheading:1348695-Phosphorylation, pubmed-meshheading:1348695-Protein Kinase C, pubmed-meshheading:1348695-Proto-Oncogene Proteins c-myc, pubmed-meshheading:1348695-RNA, Messenger, pubmed-meshheading:1348695-Staurosporine, pubmed-meshheading:1348695-Tetradecanoylphorbol Acetate, pubmed-meshheading:1348695-Tumor Cells, Cultured, pubmed-meshheading:1348695-Tyrosine 3-Monooxygenase
pubmed:year
1992
pubmed:articleTitle
Staurosporine induces a neuronal phenotype in SH-SY5Y human neuroblastoma cells that resembles that induced by the phorbol ester 12-O-tetradecanoyl phorbol-13 acetate (TPA).
pubmed:affiliation
Department of Biochemistry and Pharmacy, Abo Akademi University, Turku, Finland.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't