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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1992-4-7
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pubmed:abstractText |
We have constructed a large library of random peptides fused to the C terminus of the lac repressor. The DNA binding activity of the repressor protein physically links the peptides to the plasmid encoding them by binding to lac operator sequences on the plasmid. This linkage allows efficient enrichment for specific peptide ligands in the random population of peptides by affinity purification of the peptide-repressor-plasmid complexes with an immobilized receptor. After transformation of Escherichia coli with recovered plasmids, the library can be amplified for additional rounds of affinity enrichment or specific plasmids can be sequenced to determine the primary structure of the peptides. We used a monoclonal antibody specific for the peptide dynorphin B as a model receptor to screen a random dodecamer library. After only two rounds of enrichment, the majority of the plasmids in the selected population encoded fusion peptides that bound specifically to the antibody. These peptides contain a consensus sequence similar to a segment of dynorphin B (RQFKVV). This technique should be useful to find peptide ligands for a variety of biological receptors.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1347427-1696028,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1347427-1697402,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1347427-1990438,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1347427-1995348,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1347427-2003927,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1347427-2022328,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1347427-2143033,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1347427-2157024,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1347427-2162473,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1347427-2192357,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1347427-2199797,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1347427-2200121,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1347427-2201029,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1347427-2218529,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1347427-2768253,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1347427-2860615,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1347427-3015603,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1347427-3016526,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1347427-3041370,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1347427-3090685,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1347427-3143112,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1347427-3149606,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1347427-3301328,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1347427-3535879,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1347427-3667591,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1347427-3667592,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1347427-4599764,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1347427-6316325,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1347427-6369330,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1347427-790390
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
89
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1865-9
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pubmed:dateRevised |
2010-9-7
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pubmed:meshHeading |
pubmed-meshheading:1347427-Amino Acid Sequence,
pubmed-meshheading:1347427-Antibodies, Monoclonal,
pubmed-meshheading:1347427-Base Sequence,
pubmed-meshheading:1347427-Cloning, Molecular,
pubmed-meshheading:1347427-Dynorphins,
pubmed-meshheading:1347427-Gene Library,
pubmed-meshheading:1347427-Ligands,
pubmed-meshheading:1347427-Molecular Sequence Data,
pubmed-meshheading:1347427-Oligodeoxyribonucleotides,
pubmed-meshheading:1347427-Peptides,
pubmed-meshheading:1347427-Plasmids,
pubmed-meshheading:1347427-Receptors, Cell Surface,
pubmed-meshheading:1347427-Recombinant Fusion Proteins,
pubmed-meshheading:1347427-Repressor Proteins
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pubmed:year |
1992
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pubmed:articleTitle |
Screening for receptor ligands using large libraries of peptides linked to the C terminus of the lac repressor.
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pubmed:affiliation |
Affymax Research Institute, Palo Alto, CA 94304.
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pubmed:publicationType |
Journal Article
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