Linked Life Data

1343690

Source:http://linkedlifedata.com/resource/pubmed/id/1343690

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1994-3-11
pubmed:abstractText
The diagnosis of tick-borne diseases such as babesiosis still depends on observing the parasite in the infected erythrocyte. Microscopic observation is tedious and often problematic in both early and carrier infections. Better diagnostic methods are needed to prevent clinical disease, especially when susceptible cattle are being moved into disease enzootic areas. This study evaluates two techniques for early diagnosis of Babesia bovis infections in cattle, DNA probes specific for the organism and fluorescent probes specific for nucleic acid. The radioisotopically labeled DNA probes are used in slot blot hybridizations with lysed blood samples, not purified DNA. Thusfar, the probe is specific for B. bovis and can detect as few as 1000 B. bovis parasites in 10 microliters of blood. The specificity of the fluorescent probe depends on the characteristic morphology of the babesia in whole blood samples, as determined microscopically. The fluorescent probe detects as few as 10,000 B. bovis parasites in 10 microliters os blood. The application of each method for laboratory and field use is discussed.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0074-0276
pubmed:author
pubmed:issnType
Print
pubmed:volume
87 Suppl 3
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
193-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Non-immunologic methods of diagnosis of babesiosis.
pubmed:affiliation
Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A&M University, College Station 77843-4467.
pubmed:publicationType
Journal Article, Comparative Study, Review